CitG

• Description: fumarase
  
  

Gene name	citG
Synonyms
Essential	no
Product	fumarate hydratase
Function	TCA cycle
MW, pI	50 kDa, 5.475
Gene length, protein length	1386 bp, 462 aa
Immediate neighbours	gerAA, yuxN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU33040

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-malate = fumarate + H₂O (according to Swiss-Prot)

• Protein family: Fumarase subfamily (according to Swiss-Prot)

• Paralogous protein(s): AnsB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1YFE (E.coli)

• Swiss prot entry: P07343

• KEGG entry: [3]

• E.C. number: 4.2.1.2

Expression and regulation

• Operon: citG

• Sigma factor: SigA, SigH

• Regulation: constitutive

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP718 (spec), available in Stülke lab

• Expression vector:
  • pGP1122 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380)

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed