Difference between revisions of "PyrR"
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' [[pyrR]]-[[pyrP]]-[[pyrB]]-[[pyrC]]-[[pyrAA]]-[[pyrAB]]-[[pyrK]]-[[pyrD]]-[[pyrF]]-[[pyrE]] |
| − | * '''[[Sigma factor]]:''' | + | * '''[[Sigma factor]]:''' [[SigA]] |
| − | * '''Regulation:''' | + | * '''Regulation:''' induced in the absence of uridine nucleotides ([[PyrR]]) |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[PyrR]]: [[RNA switch]], transcription termination/ antitermination (in the presence of uridine nucleotides: termination, in their absence: antitermination) | ||
| − | * '''Additional information:''' | + | * '''Additional information:''' |
=Biological materials = | =Biological materials = | ||
Revision as of 19:51, 8 June 2009
- Description: transcriptional antiterminator of the pyr operon
| Gene name | pyrR |
| Synonyms | |
| Essential | no |
| Product | transcriptional antiterminator with minor uracil phosphoribosyltransferase activity |
| Function | regulation of pyrimidine biosynthesis |
| MW, pI | 20 kDa, 4.991 |
| Gene length, protein length | 543 bp, 181 aa |
| Immediate neighbours | ylyB, pyrP |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU15470
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)
- Protein family: PyrR subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure: 1A3C (dimeric form)
- Swiss prot entry: P39765
- KEGG entry: [3]
- E.C. number: 2.4.2.9
Additional information
Expression and regulation
- Regulation: induced in the absence of uridine nucleotides (PyrR)
- Regulatory mechanism:
- PyrR: RNA switch, transcription termination/ antitermination (in the presence of uridine nucleotides: termination, in their absence: antitermination)
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Tomchik, D. R., Turner, R. J., Switzer, R. L., and Smith, J. L. (1998) Adaptation of an enzyme to regulatory function: structure of Bacillus subtilis PyrR, a pyr RNA-binding attenuation protein and uracil phosphoribosyltransferase. Structure 6: 337-350. PubMed
- Turner, R. J., Bonner, E. R., Grabner, G. K., and Switzer, R. L. (1998) Purification and characterization of Bacillus subtilis PyrR, a bifunctional pyr mRNA-binding attenuation protein/uracil phosphoribosyltransferase. J Biol Chem 273: 5932-5938. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
