Difference between revisions of "Sandbox"

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* '''Description:''' glucomannan-specific phosphotransferase system, EIIA component <br/><br/>
+
* '''Description:''' phospho-beta-mannosidase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''gmuA''
+
|''gmuD''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ydhN''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ydhP''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || glucomannan-specific phosphotransferase system, EIIA component
+
|style="background:#ABCDEF;" align="center"| '''Product''' || phospho-beta-mannosidase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || glucomannan uptake and phosphorylation
+
|style="background:#ABCDEF;" align="center"|'''Function''' || glucomannan utilization
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 12 kDa, 5.63  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 54 kDa, 5.732  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 330 bp, 110 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1395 bp, 465 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gmuB]]'', ''[[gmuC]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gmuC]]'', ''[[gmuR]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12401&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12403&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|-
+
|colspan="2" | '''Genetic context''' <br/> [[Image:ydhP_context.gif]]
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ydhN_context.gif]]
 
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 37: Line 35:
 
=== Basic information ===
 
=== Basic information ===
  
* '''Locus tag:''' BSU05820
+
* '''Locus tag:''' BSU05840
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
Line 43: Line 41:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' no entry
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gmuBACDREFG.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12191]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12193]
  
 
=== Additional information===
 
=== Additional information===
Line 56: Line 54:
 
* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
* '''Protein family:''' PTS permease, lactose permease (Lac) family [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed]
+
* '''Protein family:'''
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
Line 82: Line 80:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU05820]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU05840]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''

Revision as of 14:05, 8 June 2009

  • Description: phospho-beta-mannosidase

Gene name gmuD
Synonyms ydhP
Essential no
Product phospho-beta-mannosidase
Function glucomannan utilization
MW, pI 54 kDa, 5.732
Gene length, protein length 1395 bp, 465 aa
Immediate neighbours gmuC, gmuR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YdhP context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU05840

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Reizer et al. (1999) Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis. Microbiology 145: 3419-3429 PubMed
  2. Sadaie Y, Nakadate H, Fukui R, Yee LM, Asai K. (2008) Glucomannan utilization operon of Bacillus subtilis. FEMS Microbiol Lett. Feb;279(1): 103-9. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed