Difference between revisions of "Sandbox"
| Line 1: | Line 1: | ||
| − | * '''Description:''' | + | * '''Description:''' xylan beta-1,4-xylosidase <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Gene name''' | |style="background:#ABCDEF;" align="center"|'''Gene name''' | ||
| − | |'' | + | |''xynB'' |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' '' | + | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ynaK '' |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || xylan beta-1,4-xylosidase |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || xylan degradation |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || | + | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 61 kDa, 5.326 |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || | + | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1599 bp, 533 aa |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ | + | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[xynP]]'', ''[[xylR]]'' |
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS: | + | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13642]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' |
|- | |- | ||
| − | |colspan="2" | '''Genetic context''' <br/> [[Image: | + | |- |
| + | |- | ||
| + | |colspan="2" | '''Genetic context''' <br/> [[Image:xynB_context.gif]] | ||
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | ||
|- | |- | ||
| Line 29: | Line 31: | ||
__TOC__ | __TOC__ | ||
| − | <br/><br/> | + | <br/><br/><br/> |
=The gene= | =The gene= | ||
| Line 35: | Line 37: | ||
=== Basic information === | === Basic information === | ||
| − | * '''Locus tag:''' | + | * '''Locus tag:''' BSU17580 |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| Line 41: | Line 43: | ||
=== Database entries === | === Database entries === | ||
| − | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ | + | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/xynPB.html] |
| − | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+ | + | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11987] |
=== Additional information=== | === Additional information=== | ||
| Line 52: | Line 54: | ||
=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' | + | * '''Catalyzed reaction/ biological activity:''' Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini (according to Swiss-Prot) |
| − | * '''Protein family:''' | + | * '''Protein family:''' glycosyl hydrolase 43 family (according to Swiss-Prot) |
* '''Paralogous protein(s):''' | * '''Paralogous protein(s):''' | ||
| Line 72: | Line 74: | ||
* '''Interactions:''' | * '''Interactions:''' | ||
| − | * '''Localization:''' | + | * '''Localization:''' cell membrane (according to Swiss-Prot) |
=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' | + | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1YIF 1YIF] |
| − | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/ | + | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P94489 P94489] |
| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+ | + | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU17580] |
| − | * '''E.C. number:''' | + | * '''E.C. number:''' [http://www.expasy.org/enzyme/3.2.1.37 3.2.1.37] |
=== Additional information=== | === Additional information=== | ||
| Line 88: | Line 90: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[ | + | * '''Operon:''' ''[[xynP]]-[[xynB]]'' |
| − | * '''[[Sigma factor]]:''' [[ | + | * '''[[Sigma factor]]:''' [[SigA]] |
| − | * '''Regulation:''' | + | * '''Regulation:''' repressed by glucose ([[CcpA]]) , carbon catabolite repression ([[CcpA]]), induction by xylose ([[XylR]]) |
| − | * '''Regulatory mechanism:''' | + | * '''Regulatory mechanism:''' [[CcpA]]: transcription repression, [[CcpA]]: transcription repression, [[XylR]]: transcription repression |
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 118: | Line 120: | ||
=References= | =References= | ||
| − | <pubmed> | + | <pubmed>8012596, </pubmed> |
| − | # | + | |
| − | # Author1, Author2 & Author3 (year) Title '' | + | # Lindner, C., Stülke, J. & Hecker, M. (1994) Regulation of xylanolytic enzymes in Bacillus subtilis. Microbiology 140: 753-757. [http://www.ncbi.nlm.nih.gov/sites/entrez/8012596 PubMed] |
| + | # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] | ||
Revision as of 13:41, 8 June 2009
- Description: xylan beta-1,4-xylosidase
| Gene name | xynB |
| Synonyms | ynaK |
| Essential | no |
| Product | xylan beta-1,4-xylosidase |
| Function | xylan degradation |
| MW, pI | 61 kDa, 5.326 |
| Gene length, protein length | 1599 bp, 533 aa |
| Immediate neighbours | xynP, xylR |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU17580
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini (according to Swiss-Prot)
- Protein family: glycosyl hydrolase 43 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure: 1YIF
- Swiss prot entry: P94489
- KEGG entry: [3]
- E.C. number: 3.2.1.37
Additional information
Expression and regulation
- Regulation: repressed by glucose (CcpA) , carbon catabolite repression (CcpA), induction by xylose (XylR)
- Regulatory mechanism: CcpA: transcription repression, CcpA: transcription repression, XylR: transcription repression
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
C Lindner, J Stülke, M Hecker
Regulation of xylanolytic enzymes in Bacillus subtilis.
Microbiology (Reading): 1994, 140 ( Pt 4);753-7
[PubMed:8012596]
[WorldCat.org]
[DOI]
(P p)
