Difference between revisions of "DegQ"
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=== Basic information === | === Basic information === | ||
| − | * '''Locus tag:''' | + | * '''Locus tag:''' BSU31720 |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| Line 82: | Line 82: | ||
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/Q99039 Q99039] | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/Q99039 Q99039] | ||
| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+ | + | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU31720] |
* '''E.C. number:''' | * '''E.C. number:''' | ||
Revision as of 11:29, 3 June 2009
- Description: stimulates production of degradative enzymes and of extracellular poly-gamma-glutamate PubMed, stimulates phosphorylation of DegU by DegS PubMed, gene is not expressed in the lab strain 168 due to promoter down mutation in the -10 region (T - 10 ---> C) PubMed
| Gene name | degQ |
| Synonyms | sacQ |
| Essential | no |
| Product | pleiotropic regulator |
| Function | regulation of exoenzyme synthesis |
| MW, pI | 5 kDa, 6.536 |
| Gene length, protein length | 138 bp, 46 aa |
| Immediate neighbours | comQ, yuzC |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU31720
Phenotypes of a mutant
loss of poly-gamma-glutamate production PubMed, no synthesis of nonribosomal peptides iturin A and plipastatin PubMed1 PubMed2, reduced synthesis of degradative enzymes PubMed, increased genetic competence PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: degQ family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure:
- Swiss prot entry: Q99039
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation: expression is induced by high cell density (ComA) PubMed,subject to carbon catabolite repression PubMed, controlled by DegU PubMed
- Regulatory mechanism: ComA: transcription activation, PubMed, DegU: transcription activation, PubMed
- Additional information: the gene is not expressed in the lab strain 168 due to promoter down mutation in the -10 region (T - 10 ---> C) PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Nobuchika Takesue, Teruo Sone, Michiko Tanaka, Fusao Tomita, Kozo Asano
Effect of an additionally introduced degQ gene on di-D-fructofuranosyl 2,6':2',6 anhydride (DFA IV) production by recombinant Bacillus subtilis in a single culture production system.
J Biosci Bioeng: 2009, 107(6);623-9
[PubMed:19447339]
[WorldCat.org]
[DOI]
(I p)
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
