Difference between revisions of "YqiD"
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| − | * '''Description:''' | + | * '''Description:''' Similar to an ''E. coli'' protein called IspA which is a fanrasyl pyrophosphate synthase <br/><br/> |
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=== Basic information === | === Basic information === | ||
| − | * '''Coordinates:''' | + | * '''Coordinates:''' 2525096-2525911 |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| Line 48: | Line 48: | ||
=== Additional information=== | === Additional information=== | ||
| + | |||
| + | Farnasyl pyrophosphate is 15 carbon compound (C15) that is an intermediate in the isoprenoid pathway. In ''B. subtilis'' there are two major isoprenoids, the synthesis of both require farnasyl pyrophosphate. The first is heptapreyl pyrophosphate (C35) which acts as a membrane anchor in menaquinone, an electron acceptor in the electron transport chain. The second is undecaprenyl pyrophosphate (C55) which is required to transport both the peptidoglycan precursor, Lipid II, and teichoic acid across the membrane. | ||
| Line 54: | Line 56: | ||
=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' | + | * '''Catalyzed reaction/ biological activity:''' dimethylallyl pyrophosphate + 2 isopentanyl pyrophosphate -> farnasyl pyrophosphate |
* '''Protein family:''' | * '''Protein family:''' | ||
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* '''Domains:''' | * '''Domains:''' | ||
| − | * '''Modification:''' | + | * '''Modification:''' None |
| − | * '''Cofactor(s):''' | + | * '''Cofactor(s):''' Magnesium |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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* '''Structure:''' | * '''Structure:''' | ||
| + | ** 1RQI: IspA from ''E. coli''. Co-crystal with substrate isopentyl pyrophosphate and substrate analogue dimethyallyl S-thiolodiphosphate [http://www.ncbi.nlm.nih.gov/pubmed/14672944 PubMed] | ||
* '''Swiss prot entry:''' | * '''Swiss prot entry:''' | ||
| − | * '''KEGG entry:''' | + | * '''KEGG entry:''' K00795 |
* '''E.C. number:''' | * '''E.C. number:''' | ||
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=Your additional remarks= | =Your additional remarks= | ||
| + | |||
| + | All information about this gene is based on its sequence similarity to the IspA protein of ''E. coli''. There is no published data on the activity of the ''B. subtilis'' protein. | ||
=References= | =References= | ||
| − | # | + | #Hosfield DJ, Zhang Y, Dougan DR, Broun A, Tari LW, Swanson RV & Finn J (2004) Structural basis for bisphosphonate-mediated inhibition of isoprenoid biosynthesis Journal of Biological Chemistry '''279(10)''': 8526-9 [http://www.ncbi.nlm.nih.gov/pubmed/14672944 PubMed] |
Revision as of 14:56, 8 March 2009
- Description: Similar to an E. coli protein called IspA which is a fanrasyl pyrophosphate synthase
| Gene name | yqiD |
| Synonyms | |
| Essential | no |
| Product | |
| Function | unknown |
| MW, pI | 29 kDa, 5.021 |
| Gene length, protein length | 816 bp, 272 aa |
| Immediate neighbours | dxs, yqiC |
| Gene sequence (+200bp) | Protein sequence |
| Caution: The sequence for this gene in SubtiList contains errors | |
Genetic context 
| |
Contents
The gene
Basic information
- Coordinates: 2525096-2525911
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
Farnasyl pyrophosphate is 15 carbon compound (C15) that is an intermediate in the isoprenoid pathway. In B. subtilis there are two major isoprenoids, the synthesis of both require farnasyl pyrophosphate. The first is heptapreyl pyrophosphate (C35) which acts as a membrane anchor in menaquinone, an electron acceptor in the electron transport chain. The second is undecaprenyl pyrophosphate (C55) which is required to transport both the peptidoglycan precursor, Lipid II, and teichoic acid across the membrane.
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: dimethylallyl pyrophosphate + 2 isopentanyl pyrophosphate -> farnasyl pyrophosphate
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: None
- Cofactor(s): Magnesium
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- 1RQI: IspA from E. coli. Co-crystal with substrate isopentyl pyrophosphate and substrate analogue dimethyallyl S-thiolodiphosphate PubMed
- Swiss prot entry:
- KEGG entry: K00795
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
All information about this gene is based on its sequence similarity to the IspA protein of E. coli. There is no published data on the activity of the B. subtilis protein.
References
- Hosfield DJ, Zhang Y, Dougan DR, Broun A, Tari LW, Swanson RV & Finn J (2004) Structural basis for bisphosphonate-mediated inhibition of isoprenoid biosynthesis Journal of Biological Chemistry 279(10): 8526-9 PubMed
