Difference between revisions of "KinB"
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** autophosphorylation, phosphorylation of [[Spo0F]] | ** autophosphorylation, phosphorylation of [[Spo0F]] | ||
** mainly active in the older, inner regions of a colony (with [[KinA]]) {{PubMed|21097618}} | ** mainly active in the older, inner regions of a colony (with [[KinA]]) {{PubMed|21097618}} | ||
| + | ** sensing potassium level in the medium during initiation of [[sliding]] [[sliding]] {{PubMed|26152584}} | ||
* '''Protein family:''' | * '''Protein family:''' | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' six transmembrane segments, C-terminal histidine phosphotransferase domain | + | * '''Domains:''' |
| + | ** six transmembrane segments, C-terminal histidine phosphotransferase domain | ||
| + | ** selectivity filter sequence of potassium channels that is required for [[sliding]] but not for [[sporulation]] {{PubMed|26152584}} | ||
* '''Modification:''' autophosphorylation on a His residue | * '''Modification:''' autophosphorylation on a His residue | ||
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* '''Mutant:''' | * '''Mutant:''' | ||
| + | ** JH19980 (''kinB''::''tet'') {{PubMed|9334321}} | ||
* '''Expression vector:''' | * '''Expression vector:''' | ||
| Line 153: | Line 157: | ||
=References= | =References= | ||
| − | <pubmed>8576055,16166384,9299348,8497199,10094672,9426145,11069677,12618455,11902725,12618455,7592498, 21097618 19101565 23378509 23599347 26152584</pubmed> | + | <pubmed>26152584,8576055,16166384,9299348,8497199,10094672,9426145,11069677,12618455,11902725,12618455,7592498, 21097618 19101565 23378509 23599347 26152584</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 11:10, 23 July 2015
- Description: two-component sensor kinase, phosphorylates Spo0F, part of the phosphorelay, senses changes in respiratory activity
| Gene name | kinB |
| Synonyms | |
| Essential | no |
| Product | two-component sensor kinase |
| Function | initiation of sporulation |
| Gene expression levels in SubtiExpress: kinB | |
| Interactions involving this protein in SubtInteract: KinB | |
| Function and regulation of this protein in SubtiPathways: kinB | |
| MW, pI | 47 kDa, 6.682 |
| Gene length, protein length | 1287 bp, 429 aa |
| Immediate neighbours | patB, kapB |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
protein modification, transcription factors and their control, phosphorelay, membrane proteins, phosphoproteins
This gene is a member of the following regulons
CodY regulon, stringent response
The gene
Basic information
- Locus tag: BSU31450
Phenotypes of a mutant
Database entries
- BsubCyc: BSU31450
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- six transmembrane segments, C-terminal histidine phosphotransferase domain
- selectivity filter sequence of potassium channels that is required for sliding but not for sporulation PubMed
- Modification: autophosphorylation on a His residue
- Cofactor(s):
- Effectors of protein activity:
- activity is triggered at low respiratory activity, this depends on a functional interaction with the respiration apparatus PubMed
- Localization: cell membrane (integral membrane protein) PubMed
Database entries
- BsubCyc: BSU31450
- UniProt: Q08430
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- repressed during growth in the presence of branched chain amino acids (CodY) PubMed
- induced upon addition of decoyinine (positive stringent response) PubMed
- Additional information:
Biological materials
- Mutant:
- JH19980 (kinB::tet) PubMed
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
