ywlE

ywlE
168

protein arginine phosphatase

Locus
BSU_36930
Molecular weight
16.64 kDa
Isoelectric point
6.68
Protein length
150 aaSequenceBlast
Gene length
453 bpSequenceBlast
Function
dephosphorylation of McsB
Product
protein arginine phosphatase
Essential
no
E.C.
3.1.3.48
Synonyms
ywlE, ipc-31d
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0394 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,791,805  3,792,257
Phenotypes of a mutant
impaired spore germination PubMed
The protein
Catalyzed reaction/ biological activity
Protein arginine phosphate + H2O --> protein arginine + phosphate PubMed
Protein family
low molecular weight phosphotyrosine protein phosphatase family (with ArsC and YfkJ, according to UniProt)
Structure
1ZGG (PDB)
P39155 (AlphaFold)
Effectors of protein activity
activity is inhibited by oxidative stress PubMed
Paralogous protein(s)
YfkJ
Localization
weak oligomerization, results in inactivation of YwlE PubMed
Expression and Regulation
Operons
Genes
ywlD-ywlE-ywlF-ywlG
Description
PubMed
Open in new tab

ywlD→ywlG

2025-03-17 20:10:54

Jstuelk

130

78d3ea170d5a096ed935a0bae51120c6b96770c1

58F865E725DD290FE4DA109529748B35ABC8310F

Genes
ywlE-ywlF-ywlG
Description
PubMed
Open in new tab

ywlE→ywlG

2025-03-30 20:44:32

Jstuelk

97

4e928c421cc36003d43336f1b2a8ad6da5bb87e9

EE90CA1B239C34BDD25117ADD762430875D6B755

Biological materials
Mutant
MGNA-A186 (ywlE::erm), available at the NBRP B. subtilis, Japan
ywlE::aphA3 available from Ulf Gerth's lab
ywlE::tet available from Ulf Gerth's lab
ywlE::spec available from Ulf Gerth's lab
GP1458 (ywlE::aphA3), available in Jörg Stülke's lab
GP1459 (ywlE::tet), available in Jörg Stülke's and Fabian Commichau's labs PubMed
BKE36930 (ywlE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTCAGTCACCCCTTATT,  downstream forward: _UP4_TAAGTTGTCAGAAAATCTGC
BKK36930 (ywlE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTCAGTCACCCCTTATT,  downstream forward: _UP4_TAAGTTGTCAGAAAATCTGC
Expression vectors
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Ulf Gerth's lab
expression of native ywlE in B. subtilis: pBP183 (in pBQ200), available in Fabian Commichau's lab
Antibody
available in Ulf Gerth's lab
Labs working on this gene/protein
Ulf Gerth, Greifswald, Germany
Fabian Commichau Göttingen, Germany homepage
References
Reviews
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Original Publications
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1BAD28287257F82F907706AFD2E8FF5F3BD1B57B

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Time of last update: 2025-04-02 11:32:31

Author of last update: Jstuelk