YtvA

• Description: blue light sensor, positive regulation of SigB activity under conditions of blue light
  
  

Gene name	ytvA
Synonyms
Essential	no
Product	blue light receptor, flavoprotein
Function	control of SigB activity
Gene expression levels in SubtiExpress: ytvA
Interactions involving this protein in SubtInteract: YtvA
MW, pI	29 kDa, 4.69
Gene length, protein length	783 bp, 261 aa
Immediate neighbours	ytvB, yttB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sigma factors and their control

This gene is a member of the following regulons

Spx regulon

The gene

Basic information

• Locus tag: BSU30340

Database entries

• BsubCyc: BSU30340

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • mediates light-dependent stimulation of RsbT kinase activity PubMed
• Protein family:

• Paralogous protein(s): RsbR, RsbRB, RsbRC, RsbRD, YtvA

Extended information on the protein

• Kinetic information:

• Domains:
  • PAS domain
  • LOV domain, STAS domain

• Modification:

• Cofactor(s): FMN

• Effectors of protein activity:
  • YtvA-dependent light activation of the SigB stress response is positively and negatively regulated by RsbRA and RsbRB, respectively PubMed

• Interactions:
  • forms dimers PubMed
  • YtvA-RsbRA PubMed
  • YtvA-RsbRD PubMed
  • part of the stressosome PubMed

• Localization:

Database entries

• BsubCyc: BSU30340

• Structure: 2PR5 (dark), 2PR6 (light)

• UniProt: O34627

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: ytvA PubMed

• Sigma factor:

• Regulation:
  • induced under conditions of disulfide stress (Spx) PubMed

• Regulatory mechanism:
  • Spx: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 42 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 127 PubMed

Biological materials

• Mutant:
  • 1A965 ( ytvA::erm), PubMed, available at BGSC

• Expression vector:
  • pGP2685, expression of His-YtvA in E. coli (based on pGP570), available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Chet Price, Davis, USA homepage

References

Reviews

Julien Herrou, Sean Crosson
Function, structure and mechanism of bacterial photosensory LOV proteins.
Nat Rev Microbiol: 2011, 9(10);713-23
[PubMed:21822294] [WorldCat.org] [DOI] (I e)

Erin B Purcell, Sean Crosson
Photoregulation in prokaryotes.
Curr Opin Microbiol: 2008, 11(2);168-78
[PubMed:18400553] [WorldCat.org] [DOI] (P p)