Difference between revisions of "MotA"

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* loss of swimming and swarming motility {{PubMed|24296669}}
 
* loss of swimming and swarming motility {{PubMed|24296669}}
 
* mucoid phenotype due to the [[DegU]]-P activated overexpression of the ''[[capB]]-[[capC]]-[[capA]]-[[capE]]'' operon and resulting overproduction of poly-gamma-glutamate {{PubMed|24296669}}
 
* mucoid phenotype due to the [[DegU]]-P activated overexpression of the ''[[capB]]-[[capC]]-[[capA]]-[[capE]]'' operon and resulting overproduction of poly-gamma-glutamate {{PubMed|24296669}}
 +
* not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation {{PubMed|26122431}}
  
 
=== Database entries ===
 
=== Database entries ===
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** 1A631 ( ''motA''::''erm''), {{PubMed|3015878}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A631&Search=1A631 BGSC]
 
** 1A631 ( ''motA''::''erm''), {{PubMed|3015878}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A631&Search=1A631 BGSC]
 
** 1A923 ( ''motA''::''erm''), {{PubMed|12813063}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A923&Search=1A923 BGSC]
 
** 1A923 ( ''motA''::''erm''), {{PubMed|12813063}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A923&Search=1A923 BGSC]
 +
** DS7498 (marker-less  in NCIB3610) {{PubMed|24296669}}
 +
** TB193 ''amyE''::Phy-''sfgfp'' (marker-less in NCIB3610 with constitutive expressed ''sfgfp'') {{PubMed|26122431}}
 +
** TB210 ''amyE''::Phy-''mKATE2'' (marker-less in NCIB3610 with constitutive expressed ''mKATE2'') {{PubMed|26122431}}
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
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=References=
 
=References=
<pubmed> 6313226 21821766 22821967 24296669 24771657 </pubmed>
+
<pubmed> 26122431, 6313226 21821766 22821967 24296669 24771657 </pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 13:14, 2 July 2015

  • Description: H+-coupled MotA-MotB flagellar stator

Gene name motA
Synonyms mot
Essential no
Product motility protein (flagellar motor rotation)
Function motility and chemotaxis
Gene expression levels in SubtiExpress: motA
Interactions involving this protein in SubtInteract: MotA
MW, pI 29 kDa, 5.01
Gene length, protein length 810 bp, 270 aa
Immediate neighbours motB, clpE
Sequences Protein DNA DNA_with_flanks
Genetic context
MotA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MotA expression.png















Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

  • Locus tag: BSU13690

Phenotypes of a mutant

  • loss of swimming and swarming motility PubMed
  • mucoid phenotype due to the DegU-P activated overexpression of the capB-capC-capA-capE operon and resulting overproduction of poly-gamma-glutamate PubMed
  • not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: motA family (according to Swiss-Prot)
  • Paralogous protein(s): MotP

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 100 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 613 PubMed

Biological materials

  • Mutant:
    • 1A631 ( motA::erm), PubMed, available at BGSC
    • 1A923 ( motA::erm), PubMed, available at BGSC
    • DS7498 (marker-less in NCIB3610) PubMed
    • TB193 amyE::Phy-sfgfp (marker-less in NCIB3610 with constitutive expressed sfgfp) PubMed
    • TB210 amyE::Phy-mKATE2 (marker-less in NCIB3610 with constitutive expressed mKATE2) PubMed
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References