Difference between revisions of "MotA"
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* loss of swimming and swarming motility {{PubMed|24296669}} | * loss of swimming and swarming motility {{PubMed|24296669}} | ||
* mucoid phenotype due to the [[DegU]]-P activated overexpression of the ''[[capB]]-[[capC]]-[[capA]]-[[capE]]'' operon and resulting overproduction of poly-gamma-glutamate {{PubMed|24296669}} | * mucoid phenotype due to the [[DegU]]-P activated overexpression of the ''[[capB]]-[[capC]]-[[capA]]-[[capE]]'' operon and resulting overproduction of poly-gamma-glutamate {{PubMed|24296669}} | ||
+ | * not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation {{PubMed|26122431}} | ||
=== Database entries === | === Database entries === | ||
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** 1A631 ( ''motA''::''erm''), {{PubMed|3015878}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A631&Search=1A631 BGSC] | ** 1A631 ( ''motA''::''erm''), {{PubMed|3015878}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A631&Search=1A631 BGSC] | ||
** 1A923 ( ''motA''::''erm''), {{PubMed|12813063}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A923&Search=1A923 BGSC] | ** 1A923 ( ''motA''::''erm''), {{PubMed|12813063}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A923&Search=1A923 BGSC] | ||
+ | ** DS7498 (marker-less in NCIB3610) {{PubMed|24296669}} | ||
+ | ** TB193 ''amyE''::Phy-''sfgfp'' (marker-less in NCIB3610 with constitutive expressed ''sfgfp'') {{PubMed|26122431}} | ||
+ | ** TB210 ''amyE''::Phy-''mKATE2'' (marker-less in NCIB3610 with constitutive expressed ''mKATE2'') {{PubMed|26122431}} | ||
* '''Expression vector:''' | * '''Expression vector:''' | ||
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=References= | =References= | ||
− | <pubmed> 6313226 21821766 22821967 24296669 24771657 </pubmed> | + | <pubmed> 26122431, 6313226 21821766 22821967 24296669 24771657 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 13:14, 2 July 2015
Gene name | motA |
Synonyms | mot |
Essential | no |
Product | motility protein (flagellar motor rotation) |
Function | motility and chemotaxis |
Gene expression levels in SubtiExpress: motA | |
Interactions involving this protein in SubtInteract: MotA | |
MW, pI | 29 kDa, 5.01 |
Gene length, protein length | 810 bp, 270 aa |
Immediate neighbours | motB, clpE |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
motility and chemotaxis, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU13690
Phenotypes of a mutant
- loss of swimming and swarming motility PubMed
- mucoid phenotype due to the DegU-P activated overexpression of the capB-capC-capA-capE operon and resulting overproduction of poly-gamma-glutamate PubMed
- not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed
Database entries
- BsubCyc: BSU13690
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
- A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: motA family (according to Swiss-Prot)
- Paralogous protein(s): MotP
Extended information on the protein
- Kinetic information:
- Modification:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- BsubCyc: BSU13690
- Structure:
- UniProt: P28611
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor: SigD PubMed
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- 1A631 ( motA::erm), PubMed, available at BGSC
- 1A923 ( motA::erm), PubMed, available at BGSC
- DS7498 (marker-less in NCIB3610) PubMed
- TB193 amyE::Phy-sfgfp (marker-less in NCIB3610 with constitutive expressed sfgfp) PubMed
- TB210 amyE::Phy-mKATE2 (marker-less in NCIB3610 with constitutive expressed mKATE2) PubMed
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References