DtpT

• Description: proton-dependent di- and tripeptide transporter
  
  

Gene name	dtpT
Synonyms	yclF
Essential	no
Product	peptide transporter
Function	uptake of di- and tripeptides
Gene expression levels in SubtiExpress: dtpT
MW, pI	53 kDa, 9.92
Gene length, protein length	1476 bp, 492 aa
Immediate neighbours	yclE, yclG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, utilization of nitrogen sources other than amino acids, membrane proteins, sporulation proteins

This gene is a member of the following regulons

ScoC regulon

The gene

Basic information

• Locus tag: BSU03670

Database entries

• BsubCyc: BSU03670

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: POT family of secondary (PMF-dependent) transporters

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU03670

• Structure:
  • 4IKV, the protein from Geobacillus kaustophilus (65% identity, 87% similarity), PubMed

• UniProt: P94408

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: dtpT PubMed

• Expression browser: dtpT PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed during exponential growth (ScoC) PubMed

• Regulatory mechanism:
  • ScoC: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Boris R Belitsky, Giulia Barbieri, Alessandra M Albertini, Eugenio Ferrari, Mark A Strauch, Abraham L Sonenshein
Interactive regulation by the Bacillus subtilis global regulators CodY and ScoC.
Mol Microbiol: 2015, 97(4);698-716
[PubMed:25966844] [WorldCat.org] [DOI] (I p)

Shintaro Doki, Hideaki E Kato, Nicolae Solcan, Masayo Iwaki, Michio Koyama, Motoyuki Hattori, Norihiko Iwase, Tomoya Tsukazaki, Yuji Sugita, Hideki Kandori, Simon Newstead, Ryuichiro Ishitani, Osamu Nureki
Structural basis for dynamic mechanism of proton-coupled symport by the peptide transporter POT.
Proc Natl Acad Sci U S A: 2013, 110(28);11343-8
[PubMed:23798427] [WorldCat.org] [DOI] (I p)

R Caldwell, R Sapolsky, W Weyler, R R Maile, S C Causey, E Ferrari
Correlation between Bacillus subtilis scoC phenotype and gene expression determined using microarrays for transcriptome analysis.
J Bacteriol: 2001, 183(24);7329-40
[PubMed:11717292] [WorldCat.org] [DOI] (P p)

E R Kunji, E J Smid, R Plapp, B Poolman, W N Konings
Di-tripeptides and oligopeptides are taken up via distinct transport mechanisms in Lactococcus lactis.
J Bacteriol: 1993, 175(7);2052-9
[PubMed:8458848] [WorldCat.org] [DOI] (P p)