Difference between revisions of "FloT"
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* '''GFP fusion:''' | * '''GFP fusion:''' | ||
− | ** GK38 (168 | + | ** GK38 (168 ''amyE''::P''floT-yfp'' (spc)), available in [[Daniel Lopez]]'s lab |
− | ** JS280 (3610 | + | ** JS280 (3610 ''amyE::floT-gfp''(spc)), available in [[Daniel Lopez]]'s lab |
− | ** JS153 (3610 | + | ** JS153 (3610 ''lacA::floT''-mEos2 (mls)), available in [[Daniel Lopez]]'s lab |
− | ** JS166 (3610 | + | ** JS166 (3610 ''lacA::floT''-PAmCherry (mls)), available in [[Daniel Lopez]]'s lab |
* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Daniel Lopez]]'s lab | * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Daniel Lopez]]'s lab |
Revision as of 11:37, 30 April 2015
- Description: similar to flotillin 1, orchestration of physiological processes in lipid microdomains, involved in the control of membrane fluidity, confers (together with YuaF) resistance to cefuroxime
Gene name | floT |
Synonyms | yuaG, yuaH |
Essential | no |
Product | bacterial flotillin-like protein |
Function | involved in the control of membrane fluidity |
Gene expression levels in SubtiExpress: floT | |
Interactions involving this protein in SubtInteract: FloT | |
MW, pI | 55 kDa, 5.135 |
Gene length, protein length | 1527 bp, 509 aa |
Immediate neighbours | yuaI, yuaF |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
biofilm formation, membrane dynamics, membrane proteins sporulation/ other, cell envelope stress proteins (controlled by SigM, V, W, X, Y)
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU31010
Phenotypes of a mutant
- delayed onset of sporulation, reduced sporulation frequency
- defect in motility PubMed
- reduced protein secretion PubMed
- a floT floA double mutant does not induce KinC-dependent biofilm formation upon addition of surfactin PubMed
- a floT floA double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency PubMed
- a floT floA double mutant has a sporulation defect, due to the lack of FtsH PubMed
- a floT mutant displays a defective growth under oxygen-limiting conditions PubMed
Database entries
- BsubCyc: BSU31010
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU31010
- Structure:
- UniProt: O32076
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- JS152 (markerless), available in Daniel Lopez's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- GK38 (168 amyE::PfloT-yfp (spc)), available in Daniel Lopez's lab
- JS280 (3610 amyE::floT-gfp(spc)), available in Daniel Lopez's lab
- JS153 (3610 lacA::floT-mEos2 (mls)), available in Daniel Lopez's lab
- JS166 (3610 lacA::floT-PAmCherry (mls)), available in Daniel Lopez's lab
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Daniel Lopez's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications