Difference between revisions of "FloT"
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* '''GFP fusion:''' | * '''GFP fusion:''' | ||
− | ** GK38 (168 | + | ** GK38 (168 ''amyE''::P''floT-yfp'' (spc)), available in [[Daniel Lopez]]'s lab |
− | ** JS280 (3610 | + | ** JS280 (3610 ''amyE::floT-gfp''(spc)), available in [[Daniel Lopez]]'s lab |
− | ** JS153 (3610 | + | ** JS153 (3610 ''lacA::floT''-mEos2 (mls)), available in [[Daniel Lopez]]'s lab |
− | ** JS166 (3610 | + | ** JS166 (3610 ''lacA::floT''-PAmCherry (mls)), available in [[Daniel Lopez]]'s lab |
* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Daniel Lopez]]'s lab | * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Daniel Lopez]]'s lab |
Revision as of 11:37, 30 April 2015
- Description: similar to flotillin 1, orchestration of physiological processes in lipid microdomains, involved in the control of membrane fluidity, confers (together with YuaF) resistance to cefuroxime
Gene name | floT |
Synonyms | yuaG, yuaH |
Essential | no |
Product | bacterial flotillin-like protein |
Function | involved in the control of membrane fluidity |
Gene expression levels in SubtiExpress: floT | |
Interactions involving this protein in SubtInteract: FloT | |
MW, pI | 55 kDa, 5.135 |
Gene length, protein length | 1527 bp, 509 aa |
Immediate neighbours | yuaI, yuaF |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
biofilm formation, membrane dynamics, membrane proteins sporulation/ other, cell envelope stress proteins (controlled by SigM, V, W, X, Y)
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU31010
Phenotypes of a mutant
- delayed onset of sporulation, reduced sporulation frequency
- defect in motility PubMed
- reduced protein secretion PubMed
- a floT floA double mutant does not induce KinC-dependent biofilm formation upon addition of surfactin PubMed
- a floT floA double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency PubMed
- a floT floA double mutant has a sporulation defect, due to the lack of FtsH PubMed
- a floT mutant displays a defective growth under oxygen-limiting conditions PubMed
Database entries
- BsubCyc: BSU31010
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU31010
- Structure:
- UniProt: O32076
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- JS152 (markerless), available in Daniel Lopez's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- GK38 (168 amyE::PfloT-yfp (spc)), available in Daniel Lopez's lab
- JS280 (3610 amyE::floT-gfp(spc)), available in Daniel Lopez's lab
- JS153 (3610 lacA::floT-mEos2 (mls)), available in Daniel Lopez's lab
- JS166 (3610 lacA::floT-PAmCherry (mls)), available in Daniel Lopez's lab
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Daniel Lopez's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
Johannes Schneider, Teresa Klein, Benjamin Mielich-Süss, Gudrun Koch, Christian Franke, Oscar P Kuipers, Ákos T Kovács, Markus Sauer, Daniel Lopez
Spatio-temporal remodeling of functional membrane microdomains organizes the signaling networks of a bacterium.
PLoS Genet: 2015, 11(4);e1005140
[PubMed:25909364]
[WorldCat.org]
[DOI]
(I e)
Juri Niño Bach, Marc Bramkamp
Dissecting the molecular properties of prokaryotic flotillins.
PLoS One: 2015, 10(1);e0116750
[PubMed:25635948]
[WorldCat.org]
[DOI]
(I e)
Benjamin Mielich-Süss, Johannes Schneider, Daniel Lopez
Overproduction of flotillin influences cell differentiation and shape in Bacillus subtilis.
mBio: 2013, 4(6);e00719-13
[PubMed:24222488]
[WorldCat.org]
[DOI]
(I e)
Juri Niño Bach, Marc Bramkamp
Flotillins functionally organize the bacterial membrane.
Mol Microbiol: 2013, 88(6);1205-17
[PubMed:23651456]
[WorldCat.org]
[DOI]
(I p)
Felix Dempwolff, Hanna M Wischhusen, Mara Specht, Peter L Graumann
The deletion of bacterial dynamin and flotillin genes results in pleiotrophic effects on cell division, cell growth and in cell shape maintenance.
BMC Microbiol: 2012, 12;298
[PubMed:23249255]
[WorldCat.org]
[DOI]
(I e)
Ana Yepes, Johannes Schneider, Benjamin Mielich, Gudrun Koch, Juan-Carlos García-Betancur, Kumaran S Ramamurthi, Hera Vlamakis, Daniel López
The biofilm formation defect of a Bacillus subtilis flotillin-defective mutant involves the protease FtsH.
Mol Microbiol: 2012, 86(2);457-71
[PubMed:22882210]
[WorldCat.org]
[DOI]
(I p)
Felix Dempwolff, Heiko M Möller, Peter L Graumann
Synthetic motility and cell shape defects associated with deletions of flotillin/reggie paralogs in Bacillus subtilis and interplay of these proteins with NfeD proteins.
J Bacteriol: 2012, 194(17);4652-61
[PubMed:22753055]
[WorldCat.org]
[DOI]
(I p)
Yong Heon Lee, Anthony W Kingston, John D Helmann
Glutamate dehydrogenase affects resistance to cell wall antibiotics in Bacillus subtilis.
J Bacteriol: 2012, 194(5);993-1001
[PubMed:22178969]
[WorldCat.org]
[DOI]
(I p)
Daniel López, Roberto Kolter
Functional microdomains in bacterial membranes.
Genes Dev: 2010, 24(17);1893-902
[PubMed:20713508]
[WorldCat.org]
[DOI]
(I p)
Catriona Donovan, Marc Bramkamp
Characterization and subcellular localization of a bacterial flotillin homologue.
Microbiology (Reading): 2009, 155(Pt 6);1786-1799
[PubMed:19383680]
[WorldCat.org]
[DOI]
(P p)
Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711]
[WorldCat.org]
[DOI]
(I p)
Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147]
[WorldCat.org]
[DOI]
(P p)
X Huang, A Gaballa, M Cao, J D Helmann
Identification of target promoters for the Bacillus subtilis extracytoplasmic function sigma factor, sigma W.
Mol Microbiol: 1999, 31(1);361-71
[PubMed:9987136]
[WorldCat.org]
[DOI]
(P p)