NagR

• Description: transcriptional regulator (GntR family)
  
  

Gene name	nagR
Synonyms	yvoA
Essential	no
Product	transcriptional regulator (GntR family)
Function	probably regulator of the putative nagA-nagB-yvoA operon
Gene expression levels in SubtiExpress: nagR
Metabolic function and regulation of this protein in SubtiPathways: nagR
MW, pI	27 kDa, 6.768
Gene length, protein length	729 bp, 243 aa
Immediate neighbours	nagB, yvnB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control

This gene is a member of the following regulons

NagR regulon

The NagR regulon:

• nagA-nagB-nagR, nagP

The gene

Basic information

• Locus tag: BSU35030

Database entries

• BsubCyc: BSU35030

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcription repression of nagP and of the putative nagA-nagB-nagR operon in the absence of N-acetylglycosamine PubMed, weak repression of yflG PubMed

• Protein family: GntR family of transcription factors

• Paralogous protein(s):

Extended information on the protein

• Kinetic information: K(D) for N-acetylglucosamine-6-phosphate: 1 mM PubMed

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:
  • N-acetylglucosamine-6-phosphate and glucosamine-6-phosphate act as the molecular inducers PubMed

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU35030

• Structure:
  • 2WVO PubMed
  • 4WWC (complex with palindromic operator DNA) PubMed

• UniProt: O34817

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: nagA-nagB-nagR PubMed

• Expression browser: nagR PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced in the presence of N-acetylglucosamine (NagR) PubMed

• Regulatory mechanism:
  • NagR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Simon B Fillenberg, Florian C Grau, Gerald Seidel, Yves A Muller
Structural insight into operator dre-sites recognition and effector binding in the GntR/HutC transcription regulator NagR.
Nucleic Acids Res: 2015, 43(2);1283-96
[PubMed:25564531] [WorldCat.org] [DOI] (I p)

Isabelle Gaugué, Jacques Oberto, Jacqueline Plumbridge
Regulation of amino sugar utilization in Bacillus subtilis by the GntR family regulators, NagR and GamR.
Mol Microbiol: 2014, 92(1);100-15
[PubMed:24673833] [WorldCat.org] [DOI] (I p)

Isabelle Gaugué, Jacques Oberto, Harald Putzer, Jacqueline Plumbridge
The use of amino sugars by Bacillus subtilis: presence of a unique operon for the catabolism of glucosamine.
PLoS One: 2013, 8(5);e63025
[PubMed:23667565] [WorldCat.org] [DOI] (I e)

Ralph Bertram, Sébastien Rigali, Natalie Wood, Andrzej T Lulko, Oscar P Kuipers, Fritz Titgemeyer
Regulon of the N-acetylglucosamine utilization regulator NagR in Bacillus subtilis.
J Bacteriol: 2011, 193(14);3525-36
[PubMed:21602348] [WorldCat.org] [DOI] (I p)

Marcus Resch, Emile Schiltz, Fritz Titgemeyer, Yves A Muller
Insight into the induction mechanism of the GntR/HutC bacterial transcription regulator YvoA.
Nucleic Acids Res: 2010, 38(7);2485-97
[PubMed:20047956] [WorldCat.org] [DOI] (I p)

Marcus Resch, Heide Marie Roth, Mathias Kottmair, Madhumati Sevvana, Ralph Bertram, Fritz Titgemeyer, Yves A Muller
Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of YvoA from Bacillus subtilis.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2009, 65(Pt 4);410-4
[PubMed:19342794] [WorldCat.org] [DOI] (I p)

CongHui You, HongYan Lu, Agnieszka Sekowska, Gang Fang, YiPing Wang, Anne-Marie Gilles, Antoine Danchin
The two authentic methionine aminopeptidase genes are differentially expressed in Bacillus subtilis.
BMC Microbiol: 2005, 5;57
[PubMed:16207374] [WorldCat.org] [DOI] (I e)