Difference between revisions of "CheB"
Line 46: | Line 46: | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
{{SubtiWiki regulon|[[CodY regulon]]}}, | {{SubtiWiki regulon|[[CodY regulon]]}}, | ||
+ | {{SubtiWiki regulon|[[DegU regulon]]}}, | ||
{{SubtiWiki regulon|[[SigD regulon]]}}, | {{SubtiWiki regulon|[[SigD regulon]]}}, | ||
{{SubtiWiki regulon|[[Spo0A regulon]]}} | {{SubtiWiki regulon|[[Spo0A regulon]]}} | ||
Line 125: | Line 126: | ||
** [[CodY]]: transcription repression {{PubMed|12730172}} | ** [[CodY]]: transcription repression {{PubMed|12730172}} | ||
** [[DegU]] (non-phosphorylated): transcription activation {{PubMed|17850253}} | ** [[DegU]] (non-phosphorylated): transcription activation {{PubMed|17850253}} | ||
+ | ** [[DegU]]-P: transcription repression {{PubMed|24386445}} | ||
+ | ** [[DegU]]-P in complex with [[SwrA]]: transcription activation {{PubMed|24386445}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
Line 152: | Line 155: | ||
<pubmed> 20122866 </pubmed> | <pubmed> 20122866 </pubmed> | ||
==Original publications== | ==Original publications== | ||
− | <pubmed>10632876,12123464,1905718,14731274,17850253,14651647, 9657996,8157612,15175317 11553614 21515776</pubmed> | + | <pubmed>10632876,12123464,1905718,14731274,17850253,14651647, 9657996,8157612,15175317 11553614 21515776 24386445</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 15:49, 29 December 2014
- Description: MCP-glutamate methylesterase / two-component response regulator-like
Gene name | cheB |
Synonyms | cheL |
Essential | no |
Product | MCP-glutamate methylesterase / two-component response regulator-like |
Function | motility and chemotaxis |
Gene expression levels in SubtiExpress: cheB | |
Interactions involving this protein in SubtInteract: CheB | |
MW, pI | 38 kDa, 8.119 |
Gene length, protein length | 1071 bp, 357 aa |
Immediate neighbours | flhG, cheA |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
motility and chemotaxis, phosphoproteins
This gene is a member of the following regulons
CodY regulon, DegU regulon, SigD regulon, Spo0A regulon
The gene
Basic information
- Locus tag: BSU16420
Phenotypes of a mutant
Database entries
- BsubCyc: BSU16420
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol (according to Swiss-Prot)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU16420
- Structure:
- UniProt: Q05522
- KEGG entry: [3]
- E.C. number: 3.1.1.61
Additional information
Expression and regulation
- Operon:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
Serena Mordini, Cecilia Osera, Simone Marini, Francesco Scavone, Riccardo Bellazzi, Alessandro Galizzi, Cinzia Calvio
The role of SwrA, DegU and P(D3) in fla/che expression in B. subtilis.
PLoS One: 2013, 8(12);e85065
[PubMed:24386445]
[WorldCat.org]
[DOI]
(I e)
Vincent J Cannistraro, George D Glekas, Christopher V Rao, George W Ordal
Cellular stoichiometry of the chemotaxis proteins in Bacillus subtilis.
J Bacteriol: 2011, 193(13);3220-7
[PubMed:21515776]
[WorldCat.org]
[DOI]
(I p)
Kazuo Kobayashi
Gradual activation of the response regulator DegU controls serial expression of genes for flagellum formation and biofilm formation in Bacillus subtilis.
Mol Microbiol: 2007, 66(2);395-409
[PubMed:17850253]
[WorldCat.org]
[DOI]
(P p)
H Werhane, P Lopez, M Mendel, M Zimmer, G W Ordal, L M Márquez-Magaña
The last gene of the fla/che operon in Bacillus subtilis, ylxL, is required for maximal sigmaD function.
J Bacteriol: 2004, 186(12);4025-9
[PubMed:15175317]
[WorldCat.org]
[DOI]
(P p)
Michael W Bunn, George W Ordal
Receptor conformational changes enhance methylesterase activity during chemotaxis by Bacillus subtilis.
Mol Microbiol: 2004, 51(3);721-8
[PubMed:14731274]
[WorldCat.org]
[DOI]
(P p)
Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647]
[WorldCat.org]
[DOI]
(P p)
Michael A Zimmer, Hendrik Szurmant, Michael M Saulmon, Marissa A Collins, Jason S Bant, George W Ordal
The role of heterologous receptors in McpB-mediated signalling in Bacillus subtilis chemotaxis.
Mol Microbiol: 2002, 45(2);555-68
[PubMed:12123464]
[WorldCat.org]
[DOI]
(P p)
E Karatan, M M Saulmon, M W Bunn, G W Ordal
Phosphorylation of the response regulator CheV is required for adaptation to attractants during Bacillus subtilis chemotaxis.
J Biol Chem: 2001, 276(47);43618-26
[PubMed:11553614]
[WorldCat.org]
[DOI]
(P p)
J R Kirby, T B Niewold, S Maloy, G W Ordal
CheB is required for behavioural responses to negative stimuli during chemotaxis in Bacillus subtilis.
Mol Microbiol: 2000, 35(1);44-57
[PubMed:10632876]
[WorldCat.org]
[DOI]
(P p)
W Estacio, S S Anna-Arriola, M Adedipe, L M Márquez-Magaña
Dual promoters are responsible for transcription initiation of the fla/che operon in Bacillus subtilis.
J Bacteriol: 1998, 180(14);3548-55
[PubMed:9657996]
[WorldCat.org]
[DOI]
(P p)
L M Márquez-Magaña, M J Chamberlin
Characterization of the sigD transcription unit of Bacillus subtilis.
J Bacteriol: 1994, 176(8);2427-34
[PubMed:8157612]
[WorldCat.org]
[DOI]
(P p)
D S Bischoff, G W Ordal
Sequence and characterization of Bacillus subtilis CheB, a homolog of Escherichia coli CheY, and its role in a different mechanism of chemotaxis.
J Biol Chem: 1991, 266(19);12301-5
[PubMed:1905718]
[WorldCat.org]
(P p)