citR

citR
168

transcriptional repressor of citA

Locus
BSU_09430
Molecular weight
35.44 kDa
Isoelectric point
8.67
Protein length
291 aaSequenceBlast
Gene length
876 bpSequenceBlast
Function
regulation of the minor citrate synthase
Product
transcriptional repressor (LysR family)
Essential
no
Synonyms
citR
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG0583 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, CitR regulon

Gene
Coordinates
1,020,073 → 1,020,948
The protein
Protein family
LysR family (according to UniProt)
Domains
HTH lysR-type domain (aa 1-58) (according to UniProt)
Structure
P39127 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
citR
Description
PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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citR

2025-03-10 02:52:22

ghost

74

3d09ef374b5ae159a0ab99e2fb221f6ad4f56a53

D0DA457A9E6F7F2AB81CAA485F15523974F4FFCA

Biological materials
Mutant
GP1283 Δ(''citR)::aphA3'', available in Jörg Stülke's lab
GP1753 Δ(''citR-citA)::aphA3, the resistance cassette can be cut out by introducing the cre-rekombinase into the chromosom of B. subtilis'', available in Jörg Stülke's lab
GP2360 Δ(''citR-citA)::erm, the resistance cassette can be cut out by introducing the cre-rekombinase into the chromosom of B. subtilis'', available in Jörg Stülke's lab
BKE09430 (ΔcitR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCTATTCTCCCTCTGA,  downstream forward: _UP4_TAGGAGCAACCAAAACGCCT
BKK09430 (ΔcitR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCTATTCTCCCTCTGA,  downstream forward: _UP4_TAGGAGCAACCAAAACGCCT
Expression vectors
pGP2264 (N-terminal His-tag, purification from E. coli, in pETM-11), available in Jörg Stülke's lab
pGP1023 (C-terminal Strep-tag, purification from E. coli, in pGP574), available in Jörg Stülke's lab
pGP1029 (N-terminal Strep-tag, purification from B. subtilis, in pGP380), available in Jörg Stülke's lab
pGP1030 (C-terminal Strep-tag, purification from B. subtilis, in pGP382), available in Jörg Stülke's lab
pGP2260 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
pGP2262 (integration into ganA, expression under the control of the xylose-inducible PxylA promoter in B. subtilis, in pGP888), available in Jörg Stülke's lab
References
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Page visits: 5461

Time of last update: 2025-04-05 07:52:23

Author of last update: Melvin.boenninger