Difference between revisions of "DarA"
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| − | * '''Description:''' | + | * '''Description:''' c-di-AMP-binding PII-like protein<br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Essential''' || | |style="background:#ABCDEF;" align="center"| '''Essential''' || | ||
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || c-di-AMP-binding PII-like protein |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown | |style="background:#ABCDEF;" align="center"|'''Function''' || unknown | ||
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* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| + | ** binds c-di-AMP {{PubMed|25433025}} | ||
* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
| − | ** forms homo-trimers | + | ** forms homo-trimers {{PubMed|25433025}} |
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
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* '''Structure:''' | * '''Structure:''' | ||
| − | ** [http://www.pdb.org/pdb/explore/explore.do?structureId=4RLE 4RLE] (the ''B. subtilis'' protein in complex with c-di-AMP) | + | ** [http://www.pdb.org/pdb/explore/explore.do?structureId=4RLE 4RLE] (the ''B. subtilis'' protein in complex with c-di-AMP) {{PubMed|25433025}} |
** [http://www.pdb.org/pdb/explore/explore.do?structureId=3m05 3M05] (the apoprotein from ''Pediococcus pentosaceus'', 50% identity) | ** [http://www.pdb.org/pdb/explore/explore.do?structureId=3m05 3M05] (the apoprotein from ''Pediococcus pentosaceus'', 50% identity) | ||
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=Biological materials = | =Biological materials = | ||
* '''Mutant:''' | * '''Mutant:''' | ||
| − | ** GP1712 (''[[darA]]''::''cat''), available in [[Jörg Stülke]]'s lab | + | ** GP1712 (''[[darA]]''::''cat''), available in [[Jörg Stülke]]'s lab {{PubMed|25433025}} |
** GP1713 (''[[darA]]-[[yaaR]]''::''cat''), available in [[Jörg Stülke]]'s lab | ** GP1713 (''[[darA]]-[[yaaR]]''::''cat''), available in [[Jörg Stülke]]'s lab | ||
* '''Expression vector:''' | * '''Expression vector:''' | ||
| − | **pGP2601: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]], available in [[Jörg Stülke]]'s lab | + | **pGP2601: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]], available in [[Jörg Stülke]]'s lab {{PubMed|25433025}} |
**pGP2624: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]], available in [[Jörg Stülke]]'s lab | **pGP2624: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]], available in [[Jörg Stülke]]'s lab | ||
**pGP2603: expression of ''yaaQ''-Strep by [[pGP382]] in ''B. subtilis'' suitable for [[SPINE]], available in [[Jörg Stülke]]'s lab | **pGP2603: expression of ''yaaQ''-Strep by [[pGP382]] in ''B. subtilis'' suitable for [[SPINE]], available in [[Jörg Stülke]]'s lab | ||
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=References= | =References= | ||
| − | <pubmed> 22383849</pubmed> | + | <pubmed> 22383849 25433025 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 10:28, 30 November 2014
- Description: c-di-AMP-binding PII-like protein
| Gene name | darA |
| Synonyms | yaaQ |
| Essential | |
| Product | c-di-AMP-binding PII-like protein |
| Function | unknown |
| Gene expression levels in SubtiExpress: darA | |
| MW, pI | 11 kDa, 6.088 |
| Gene length, protein length | 327 bp, 109 aa |
| Immediate neighbours | tmk, yaaR |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU00290
Phenotypes of a mutant
Database entries
- BsubCyc: BSU00290
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- binds c-di-AMP PubMed
- Interactions:
- forms homo-trimers PubMed
Database entries
- BsubCyc: BSU00290
- Structure:
- UniProt: P37538
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1030 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 922 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 894 PubMed
Biological materials
- Mutant:
- GP1712 (darA::cat), available in Jörg Stülke's lab PubMed
- GP1713 (darA-yaaR::cat), available in Jörg Stülke's lab
- Expression vector:
- pGP2601: IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab PubMed
- pGP2624: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
- pGP2603: expression of yaaQ-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody: available in Jörg Stülke's lab
Labs working on this gene/protein
Your additional remarks
References
