AlsS

• Description: acetolactate synthase
  
  

Gene name	alsS
Synonyms
Essential	no
Product	acetolactate synthase
Function	overflow metabolism
Gene expression levels in SubtiExpress: alsS
Metabolic function and regulation of this protein in SubtiPathways: alsS
MW, pI	61 kDa, 5.164
Gene length, protein length	1713 bp, 571 aa
Immediate neighbours	alsD, alsR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism

This gene is a member of the following regulons

AlsR regulon, Rex regulon, stringent response

The gene

Basic information

• Locus tag: BSU36010

Database entries

• BsubCyc: BSU36010

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2 pyruvate = 2-acetolactate + CO₂ (according to Swiss-Prot)

• Protein family: TPP enzyme family (according to Swiss-Prot)

• Paralogous protein(s): IlvB, YdaP

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU36010

• Structure:

• UniProt: Q04789

• KEGG entry: [3]

• E.C. number: 2.2.1.6

Expression and regulation

• Operon: alsS-alsD PubMed

• Expression browser: alsS PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induction by acetate (AlsR) PubMed
  • repressed as long as terminal electron acceptors are available for respiration (Rex) PubMed
  • subject to positive stringent control upon lysine starvation PubMed

Note: since acetate formation requires ackA activation by CcpA there is an indirect effect of CcpA on the alsSD operon: the operon is not expressed in ccpA mutants

• Regulatory mechanism:
  • stringent response: due to presence of adenines at +1 and +2 positions of the transcript PubMed
  • AlsR: transcription activation in the presence of acetate PubMed
  • Rex: transcription repression if the ratio NADH2/NAD is high PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A979 ( alsS::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: