WalR

• Description: two-component response regulator (OmpR family), controls cell wall metabolism
  
  

Gene name	walR
Synonyms	yycF
Essential	yes PubMed
Product	two-component response regulator (OmpR family)
Function	control of cell wall metabolism
Gene expression levels in SubtiExpress: walR
Interactions involving this protein in SubtInteract: WalR
MW, pI	27 kDa, 4.876
Gene length, protein length	705 bp, 235 aa
Immediate neighbours	walK, trnY-Phe
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall/ other, transcription factors and their control, essential genes, phosphoproteins

The gene

Basic information

• Locus tag: BSU40410

Phenotypes of a mutant

• essential PubMed
• a constitutively active form of WalR (WalR-R204C) results in constitutive expression of the WalR regulon, and in combination with a sepF mutation to the formation of cell wall-less L-forms PubMed

Database entries

• BsubCyc: BSU40410

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: OmpR family of two-component response regulators

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated by WalK on an Asp residue

• Cofactors:

• Effectors of protein activity: phosphorylation likely affects DNA-binding activity

• Interactions:
  • WalK-WalR

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU40410

• Structure: 2D1V (DNA binding domain), 2ZWM (receiver domain)

• UniProt: P37478

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: walR-walK-yycH-yycI-walJ-htrC PubMed

• Expression browser: walR PubMed

• Sigma factor:

• Regulation:
  • expressed during vegetative growth, repressed during stationary phase PubMed
  • induced at high temperature PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 176 PubMed

Biological materials

• Mutant:

• Expression vector:
  • pGP2633, constitutive expression in B. subtilis (based on pBQ200), available in Jörg Stülke's lab
  • pGP2635, expression of His-WalR in E. coli (based on pWH844), available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Kevin Devine, Dublin