Difference between revisions of "PbpB"
Line 142: | Line 142: | ||
* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jeff Errington]] lab | * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jeff Errington]] lab | ||
+ | |||
+ | * '''FLAG-tag construct:''' | ||
+ | ** GP2003 ''pbpB-3xFLAG spec'' (based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab | ||
* '''Antibody:''' | * '''Antibody:''' |
Revision as of 15:54, 15 August 2014
- Description: class B penicillin-binding protein 2B, Pbp2B
Gene name | pbpB |
Synonyms | |
Essential | yes PubMed |
Product | penicillin-binding protein 2B |
Function | septation, cell division |
Gene expression levels in SubtiExpress: pbpB | |
Interactions involving this protein in SubtInteract: PbpB | |
MW, pI | 79 kDa, 9.234 |
Gene length, protein length | 2148 bp, 716 aa |
Immediate neighbours | ftsL, spoVD |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
cell wall synthesis, cell division, essential genes, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU15160
Phenotypes of a mutant
essential PubMed
Database entries
- BsubCyc: BSU15160
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: transpeptidase family (according to Swiss-Prot)
- Paralogous protein(s): SpoVD
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
Database entries
- BsubCyc: BSU15160
- UniProt: Q07868
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- constitutively expressed PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab
- FLAG-tag construct:
- GP2003 pbpB-3xFLAG spec (based on pGP1331), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Jeff Errington, Newcastle University, UK homepage
Your additional remarks
References