RpoY

• Description: epsilon subunit of RNA polymerase
  
  

Gene name	rpoY
Synonyms	ykzG
Essential	no
Product	epsilon subunit of RNA polymerase
Function	control of RNA polymerase activity
Gene expression levels in SubtiExpress: rpoY
Interactions involving this protein in SubtInteract: RpoY
MW, pI	8 kDa, 4.605
Gene length, protein length	207 bp, 69 aa
Immediate neighbours	rnjA, ykrA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription

The gene

Basic information

• Locus tag: BSU14540

Database entries

• BsubCyc: BSU14540

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: UPF0356 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RpoC-RpoY PubMed

• Localization:
  • colocalizes with the nucleoid PubMed

Database entries

• BsubCyc: BSU14540

• Structure: 4NJC PubMed

• UniProt: O31718

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: rpoY-rnjA PubMed

• Expression browser: rpoY PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • translation of YkzG and RnjA is coupled, and this coupling is required for efficient expression of RNase J1 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 443 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion: pGP420 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody: