Difference between revisions of "TopA"

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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''topI ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''topI ''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
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|style="background:#ABCDEF;" align="center"| '''Essential''' || yes {{PubMed|25092907}}
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Product''' || DNA topoisomerase I
 
|style="background:#ABCDEF;" align="center"| '''Product''' || DNA topoisomerase I
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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[DNA condensation/ segregation]]}}
+
{{SubtiWiki category|[[DNA condensation/ segregation]]}},
 +
{{SubtiWiki category|[[essential genes]]}}
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
* essential {{PubMed|25092907}}
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Protein family:''' prokaryotic type I/III topoisomerase family (according to Swiss-Prot)
 
* '''Protein family:''' prokaryotic type I/III topoisomerase family (according to Swiss-Prot)
  
* '''Paralogous protein(s):''' [[TopB]], one of the two proteins has to be present for viability [http://www.ncbi.nlm.nih.gov/sites/entrez/17114254 PubMed]
+
* '''Paralogous protein(s):''' [[TopB]]
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
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=References=
 
=References=
<pubmed>17114254,21710567 </pubmed>
+
<pubmed>17114254,21710567 25092907</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 09:26, 6 August 2014

  • Description: DNA topoisomerase I

Gene name topA
Synonyms topI
Essential yes PubMed
Product DNA topoisomerase I
Function DNA replication
Gene expression levels in SubtiExpress: topA
MW, pI 78 kDa, 9.023
Gene length, protein length 2073 bp, 691 aa
Immediate neighbours dprA, trmFO
Sequences Protein DNA DNA_with_flanks
Genetic context
TopA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TopA expression.png















Categories containing this gene/protein

DNA condensation/ segregation, essential genes

This gene is a member of the following regulons

Efp-dependent proteins

The gene

Basic information

  • Locus tag: BSU16120

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to Swiss-Prot)
  • Protein family: prokaryotic type I/III topoisomerase family (according to Swiss-Prot)
  • Paralogous protein(s): TopB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: topA (according to DBTBS)
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 324 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 1123 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Mario Juhas, Daniel R Reuß, Bingyao Zhu, Fabian M Commichau
Bacillus subtilis and Escherichia coli essential genes and minimal cell factories after one decade of genome engineering.
Microbiology (Reading): 2014, 160(Pt 11);2341-2351
[PubMed:25092907] [WorldCat.org] [DOI] (I p)

Olivier Delumeau, François Lecointe, Jan Muntel, Alain Guillot, Eric Guédon, Véronique Monnet, Michael Hecker, Dörte Becher, Patrice Polard, Philippe Noirot
The dynamic protein partnership of RNA polymerase in Bacillus subtilis.
Proteomics: 2011, 11(15);2992-3001
[PubMed:21710567] [WorldCat.org] [DOI] (I p)

Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254] [WorldCat.org] [DOI] (P p)