YloB

• Description: similar to the E. coli MgtA magnesium transporter
  
  

Gene name	yloB
Synonyms
Essential	no
Product	unknown
Function	unknown
Gene expression levels in SubtiExpress: yloB
Metabolic function and regulation of this protein in SubtiPathways: YloB
MW, pI	97 kDa, 8.714
Gene length, protein length	2670 bp, 890 aa
Immediate neighbours	yloA, yloC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), membrane proteins, sporulation proteins

The gene

Basic information

• Locus tag: BSU15650

Database entries

• BsubCyc: BSU15650

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • ATP + H₂O + Ca²⁺(Cis) = ADP + phosphate + Ca²⁺(Trans) (according to Swiss-Prot)
  • there is no indication for an implication of YloB in Mg²⁺ uptake (based on mutant phenotypes, failure to complement mutants defective in Mg²⁺ uptake) PubMed

• Protein family: Type IIA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU15650

• Structure:

• UniProt: O34431

• KEGG entry: [2]

• E.C. number: 3.6.3.8

Expression and regulation

• Operon:

• Expression browser: yloB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Catherine A Wakeman, Jonathan R Goodson, Vineetha M Zacharia, Wade C Winkler
Assessment of the requirements for magnesium transporters in Bacillus subtilis.
J Bacteriol: 2014, 196(6);1206-14
[PubMed:24415722] [WorldCat.org] [DOI] (I p)