YukE

• Description: secreted protein of the WXG100 superfamily
  
  

Gene name	yukE
Synonyms
Essential	no
Product	unknown
Function	unknown
Gene expression levels in SubtiExpress: yukE
MW, pI	8 kDa, 7.909
Gene length, protein length	240 bp, 80 aa
Immediate neighbours	yukD, adeR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteins of unknown function, most abundant proteins

This gene is a member of the following regulons

DegU regulon

The gene

Basic information

• Locus tag: BSU31910

Database entries

• BsubCyc: BSU31910

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: WXG100 superfamily (pfam06013) PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • forms dimers PubMed

• Localization:
  • secreted, by the type VII protein secretion system YukD-YukC-YukB-YueB-YueC PubMed

Database entries

• BsubCyc: BSU31910

• Structure:

• UniProt: C0SP85

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yukE-yukD-yukC-yukB-yueB-yueC PubMed

• Expression browser: yukE PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • not expressed in the lab strain 168, due to lack of DegU-P PubMed
  • expressed in the stationary phase PubMed

• Regulatory mechanism:
  • DegU-P: transcription activation PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1775 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 23591 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 10799 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 11580 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 4748 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Catarina Baptista, Hugo Condessa Barreto, Carlos São-José
High levels of DegU-P activate an Esat-6-like secretion system in Bacillus subtilis.
PLoS One: 2013, 8(7);e67840
[PubMed:23861817] [WorldCat.org] [DOI] (I e)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Carlos São-José, Catarina Baptista, Mário A Santos
Bacillus subtilis operon encoding a membrane receptor for bacteriophage SPP1.
J Bacteriol: 2004, 186(24);8337-46
[PubMed:15576783] [WorldCat.org] [DOI] (P p)

Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759] [WorldCat.org] [DOI] (P p)

Mark J Pallen
The ESAT-6/WXG100 superfamily -- and a new Gram-positive secretion system?
Trends Microbiol: 2002, 10(5);209-12
[PubMed:11973144] [WorldCat.org] [DOI] (P p)