NupO

• Description: ABC transporter for guanosine (ATP-binding protein)
  
  

Gene name	nupO
Synonyms	yufO
Essential	no
Product	ABC transporter for guanosine
Function	uptake of guanosine
Gene expression levels in SubtiExpress: nupO
Interactions involving this protein in SubtInteract: NupO
MW, pI	56 kDa, 7.497
Gene length, protein length	1530 bp, 510 aa
Immediate neighbours	nupN, nupP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, biosynthesis/ acquisition of nucleotides, membrane proteins

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

• Locus tag: BSU31550

Phenotypes of a mutant

• a nupO nupG double mutant is unable to utilize externally provided guanosine PubMed

Database entries

• BsubCyc: BSU31550

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ABC transporter family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • NupO-(NupP-NupQ) PubMed

• Localization: associated to the membrane (via NupP-NupQ) PubMed

Database entries

• BsubCyc: BSU31550

• Structure:

• UniProt: O05253

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: nupN-nupO-nupP-nupQ PubMed

• Expression browser: nupO PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1042 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 504 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 562 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: