Difference between revisions of "DarA"
| Line 114: | Line 114: | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| + | ** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1030 {{PubMed|21395229}} | ||
| + | ** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 922 {{PubMed|21395229}} | ||
| + | ** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 894 {{PubMed|21395229}} | ||
=Biological materials = | =Biological materials = | ||
| − | |||
* '''Mutant:''' | * '''Mutant:''' | ||
** GP1712 (''[[yaaQ]]''::''cat''), available in [[Jörg Stülke]]'s lab | ** GP1712 (''[[yaaQ]]''::''cat''), available in [[Jörg Stülke]]'s lab | ||
Revision as of 14:16, 17 April 2014
- Description: unknown
| Gene name | yaaQ |
| Synonyms | |
| Essential | |
| Product | unknown |
| Function | unknown |
| Gene expression levels in SubtiExpress: yaaQ | |
| MW, pI | 11 kDa, 6.088 |
| Gene length, protein length | 327 bp, 109 aa |
| Immediate neighbours | tmk, yaaR |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU00290
Phenotypes of a mutant
Database entries
- BsubCyc: BSU00290
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Interactions:
- forms homo-trimers (see 3M05)
Database entries
- BsubCyc: BSU00290
- Structure:
- 3M05 (the protein from Pediococcus pentosaceus, 50% identity)
- UniProt: P37538
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1030 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 922 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 894 PubMed
Biological materials
- Mutant:
- GP1712 (yaaQ::cat), available in Jörg Stülke's lab
- GP1713 (yaaQ-yaaR::cat), available in Jörg Stülke's lab
- Expression vector:
- pGP2601: IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab
- pGP2603: expression of yaaQ-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
