rocF

rocF
168

arginase

Locus
BSU_40320
Molecular weight
32.00 kDa
Isoelectric point
4.93
Protein length
296 aaSequenceBlast
Gene length
891 bpSequenceBlast
Function
arginine utilization
Product
arginase
Essential
no
E.C.
3.5.3.1
Synonyms
rocF, RocF
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0010 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,141,711 → 4,142,601
Phenotypes of a mutant
unable to grow with arginine as single source of nitrogen PubMed
The protein
Catalyzed reaction/ biological activity
H2O + L-arginine --> L-ornithine + urea (according to UniProt)
Protein family
arginase family (with SpeB and HutG, according to UniProt)
Cofactors
Mn2+ PubMed
Structure
6NFP (PDB)
6DKT (PDB)
P39138 (AlphaFold)
Modification
phosphorylated on Ser-68 PubMed
Kinetic information
Km for arginine: 4.6 mM PubMed
Expression and Regulation
Operons
Genes
rocD-rocE-rocF
Description
PubMed
Regulation
induced in the presence of arginine (AhrC) PubMed
induced in the presence of arginine, citrulline or ornithine (with ornithine as the molecular inducer) (RocR) PubMed
Regulatory mechanism
Spo0A: repression, PubMed, in spo0A regulon
AhrC: activation, PubMed, in ahrC regulon
CodY: repression, PubMed, in codY regulon
RocR: activation, PubMed, in rocR regulon
Sigma factors
SigL: sigma factor, PubMed, in sigL regulon
Additional information
expression of the rocD-rocE-rocF operon is increased upon depletion of nusA (resulting from increased ahrC expression) Reference
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rocD→rocF

2025-04-04 08:12:16

Jstuelk

142

2736177DCAE8E1DC5951F68E1BB418B48A3DD8A0

67A3AD2158130CCC732BB0A6D29A52A60C79A4AD

Biological materials
Mutant
GP655, aphA3, available in Jörg Stülke's lab PubMed
BKE40320 (ΔrocF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTGTGATTCCACCTCAA,  downstream forward: _UP4_TAATAAGAAAACCCCCGCAC
BKK40320 (ΔrocF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTGTGATTCCACCTCAA,  downstream forward: _UP4_TAATAAGAAAACCCCCGCAC
Expression vectors
pGP3796: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
pGP3780: expression of Strep-rocF by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
References
Reviews
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Original Publications
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CD121BBF1DC206D1189ECF8B32456641A55C41D4

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Time of last update: 2025-04-09 21:05:23

Author of last update: Jstuelk