Gmk

• Description: guanylate kinase (GMP:dATP, dGMP:ATP)
  
  

Gene name	gmk
Synonyms	yloD
Essential	yes PubMed
Product	guanylate kinase (GMP:dATP, dGMP:ATP)
Function	GTP biosynthesis
Gene expression levels in SubtiExpress: gmk
Metabolic function and regulation of this protein in SubtiPathways: gmk
MW, pI	23 kDa, 4.616
Gene length, protein length	612 bp, 204 aa
Immediate neighbours	ylzA, yloH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, essential genes, phosphoproteins

The gene

Basic information

• Locus tag: BSU15680

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU15680

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + GMP = ADP + GDP (according to Swiss-Prot)

• Protein family: guanylate kinase-like domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-134 and/or Arg-149PubMed

• Cofactor(s):

• Effectors of protein activity:
  • inhibition of enzymatic activity by (p)ppGpp during the ´stringent response´PubMed

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU15680

• Structure: 1S4Q (from Mycobacterium tuberculosis h37rv, 39% identity, 60% similarity)

• UniProt: O34328

• KEGG entry: [2]

• E.C. number: 2.7.4.8

Expression and regulation

• Operon:

• Expression browser: gmk PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 254 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1208 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1529 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 895 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1794 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: