Ald

• Description: L-alanine dehydrogenase
  
  

Gene name	ald
Synonyms	spoVN
Essential	no
Product	L-alanine dehydrogenase
Function	alanine utilization
Gene expression levels in SubtiExpress: ald
Metabolic function and regulation of this protein in SubtiPathways: ald
MW, pI	39 kDa, 5.125
Gene length, protein length	1134 bp, 378 aa
Immediate neighbours	adeR, yukJ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

AdeR regulon

The gene

Basic information

• Locus tag: BSU31930

Phenotypes of a mutant

• sporulation defect PubMed

Database entries

• BsubCyc: BSU31930

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-alanine + H₂O + NAD⁺ = pyruvate + NH₃ + NADH (according to Swiss-Prot)

• Protein family: AlaDH/PNT family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU31930

• Structure: 2EEZ (from Thermus thermophilus hb8 mutant, 55% identity, 66% similarity)

• UniProt: Q08352

• KEGG entry: [3]

• E.C. number: 1.4.1.1

Expression and regulation

• Operon: ald PubMed

• Expression browser: ald PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by alanine (AdeR) PubMed

• Regulatory mechanism:
  • AdeR: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 845 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 74423 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 6392 PubMed

• • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 15279 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed