DegS

• Description: two-component sensor kinase for exoenzyme and competence regulation, kinase activity is activated in response to inhibition of flagellar rotation
  
  

Gene name	degS
Synonyms	sacU
Essential	no
Product	two-component sensor kinase
Function	regulation of degradative enzyme and genetic competence
Gene expression levels in SubtiExpress: degS
Interactions involving this protein in SubtInteract: DegS
MW, pI	44 kDa, 5.988
Gene length, protein length	1155 bp, 385 aa
Immediate neighbours	degU, yvyE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, transcription factors and their control, phosphoproteins

The gene

Basic information

• Locus tag: BSU35500

Phenotypes of a mutant

• the mutation suppresses the mucoid phenotype of motA or motB mutants due to loss of DegU phosphorylation and concomitant reduced expression of the capB-capC-capA-capE operon PubMed

Database entries

• BsubCyc: BSU35500

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • autophosphorylation, autodephosphorylation, phosphorylation of DegU on Asp-56 PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • aa 12 ... 170: unknown DegS domain
  • aa 180 ... 248: HisKA_3 domain (autophosphorylation)
  • aa 289 ... 384: HATPase_c

• Modification:
  • phosphorylation on Ser-76 PubMed, this stimulates phosphotransfer from His-189 to DegU PubMed, the phosphorylyation is performed by PrkD in vitro PubMed
  • autophosphorylation on His-189

• Cofactors:

• Effectors of protein activity:
  • DegQ (stimulation of autophosphorylation) PubMed
  • inhibition of flagellar rotation stimulates kinase activity PubMed

• Interactions:
  • DegS-DegU
  • DegS-DegQ PubMed

• Localization: Cytoplasm (Homogeneous) PubMed

Database entries

• BsubCyc: BSU35500

• Structure:

• UniProt: P13799

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: degS-degU PubMed

• Expression browser: degS PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 69 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Tarek Msadek, Institut Pasteur, Paris, France

References

Reviews

Robert Belas
When the swimming gets tough, the tough form a biofilm.
Mol Microbiol: 2013, 90(1);1-5
[PubMed:23927648] [WorldCat.org] [DOI] (I p)