FtsH

• Description: ATP-dependent metalloprotease
  
  

Gene name	ftsH
Synonyms
Essential	no
Product	ATP-dependent metalloprotease
Function	cell division, sporulation initiation
Gene expression levels in SubtiExpress: ftsH
Interactions involving this protein in SubtInteract: FtsH
Metabolic function and regulation of this protein in SubtiPathways: ftsH
MW, pI	70 kDa, 5.841
Gene length, protein length	1911 bp, 637 aa
Immediate neighbours	hprT, coaX
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell division, proteolysis, cell envelope stress proteins (controlled by SigM, V, W, X, Y), heat shock proteins, biofilm formation, membrane proteins

This gene is a member of the following regulons

SigM regulon, TilS-HprT regulon

The gene

Basic information

• Locus tag: BSU00690

Phenotypes of a mutant

• defective in biofilm formation PubMed
• strongly reduced sporulation frequency PubMed

Database entries

• BsubCyc: BSU00690

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • degrades Spo0E and Spo0M, resulting in reduced sporulation frequency in a ftsH mutant PubMed
  • FtsH is involved in the degradation of EzrA PubMed

• Protein family:

• Paralogous protein(s): YjoB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • protease activity is controlled by the interaction with the flottilins FloA and FloT PubMed

• Interactions:
  • SpoVM-FtsH PubMed
  • FloT-FtsH PubMed
  • FloA-FtsH PubMed
  • FtsH-Spo0E PubMed
  • FtsH-Spo0M PubMed

• Localization:
  • cell membrane PubMed
  • preferentially at the division septum PubMed
  • forms some foci in the membrane PubMed

Database entries

• BsubCyc: BSU00690

• Structure: 1LV7 (from E. coli, 64% identity) PubMed

• UniProt: P37476

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • ftsH PubMed
  • tilS-hprT-ftsH PubMed

• Expression browser: ftsH PubMed

• Sigma factor: SigA PubMed, SigM PubMed

• Regulation: induced by heat shock (class III)

• Regulatory mechanism:
  • TilS-HprT: transcription activation PubMed

• Additional information:
  • the mRNA is very stable (half-life > 15 min) PubMed
  • the FtsH levels are reduced in a floT floA double mutant PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1026 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 5042 PubMed

Biological materials

• Mutant:
  • 1A1061 ( ftsH::cat), PubMed, available at BGSC
  • 1A1060 ( ftsH::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Noël Molière, Kürşad Turgay
General and regulatory proteolysis in Bacillus subtilis.
Subcell Biochem: 2013, 66;73-103
[PubMed:23479438] [WorldCat.org] [DOI] (P p)