YfiT

• Description: bacillithiol S-transferase
  
  

Gene name	yfiT
Synonyms
Essential	no
Product	bacillithiol S-transferase
Function	detoxification
Gene expression levels in SubtiExpress: yfiT
MW, pI	20 kDa, 6.612
Gene length, protein length	534 bp, 178 aa
Immediate neighbours	yfiS, yfiU
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

resistance against oxidative and electrophile stress

The gene

Basic information

• Locus tag: BSU08390

Database entries

• BsubCyc: BSU08390

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: metal hydrolase yfiT family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): Ni(2+) PubMed

• Effectors of protein activity:

• Interactions:
  • homodimer PubMed

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU08390

• Structure: 1RXQ

• UniProt: O31562

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yfiT (according to DBTBS)

• Expression browser: yfiT PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 206 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 992 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Gerald L Newton, Stephan S Leung, Judy I Wakabayashi, Mamta Rawat, Robert C Fahey
The DinB superfamily includes novel mycothiol, bacillithiol, and glutathione S-transferases.
Biochemistry: 2011, 50(49);10751-60
[PubMed:22059487] [WorldCat.org] [DOI] (I p)

Shyamala S Rajan, Xiaojing Yang, Ludmilla Shuvalova, Frank Collart, Wayne F Anderson
YfiT from Bacillus subtilis is a probable metal-dependent hydrolase with an unusual four-helix bundle topology.
Biochemistry: 2004, 43(49);15472-9
[PubMed:15581359] [WorldCat.org] [DOI] (P p)