Difference between revisions of "PdhB"
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* '''Additional information:''' | * '''Additional information:''' | ||
** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}} | ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}} | ||
+ | ** number of protein molecules per cell (minimal medium with glucose and ammonium): 10912 {{PubMed|24696501}} | ||
+ | ** number of protein molecules per cell (complex medium with amino acids, without glucose): 30160 {{PubMed|24696501}} | ||
=Biological materials = | =Biological materials = |
Revision as of 09:41, 17 April 2014
- Description: pyruvate dehydrogenase (E1 beta subunit)
Gene name | pdhB |
Synonyms | |
Essential | no |
Product | pyruvate dehydrogenase (E1 beta subunit) |
Function | links glycolysis and TCA cycle |
Gene expression levels in SubtiExpress: pdhB | |
Interactions involving this protein in SubtInteract: PdhB | |
Metabolic function and regulation of this protein in SubtiPathways: pdhB | |
MW, pI | 35 kDa, 4.547 |
Gene length, protein length | 975 bp, 325 aa |
Immediate neighbours | pdhA, pdhC |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
carbon core metabolism, membrane proteins, phosphoproteins, most abundant proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU14590
Phenotypes of a mutant
- defects in sporulation and unable to grow on glucose as single carbon source PubMed
Database entries
- BsubCyc: BSU14590
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2 (according to Swiss-Prot)
- Protein family:
Extended information on the protein
- Kinetic information: Michaelis-Menten PubMed
- Modification: phosphorylation on (Ser-302 OR Ser-306) PubMed
- Effectors of protein activity:
- Inhibited thiamine 2-thiothiazolone diphosphate and NADH PubMed
- Low sensibility to NADPH
- Localization: membrane associated PubMed
Database entries
- BsubCyc: BSU14590
- Structure: 1W88 (E1 in complex with subunit binding domain of E2, Geobacillus stearothermophilus)
- UniProt: P21882
- KEGG entry: [3]
- E.C. number: 1.2.4.1
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- stringent response: due to presence of guanine at +1 position of the transcript PubMed
- Additional information:
- belongs to the 100 most abundant proteins PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium): 10912 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 30160 PubMed
Biological materials
- Mutant: GP459 (spc), available in Stülke lab
- Expression vector:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Arthur Aronson, Purdue University, West Lafayette, USA homepage
Your additional remarks
References
Reviews
Original publications