GroES

• Description: chaperonin, universally conserved protein
  
  

Gene name	groES
Synonyms
Essential	yes PubMed
Product	chaperonin
Function	protein folding and re-folding
Gene expression levels in SubtiExpress: groES
Interactions involving this protein in SubtInteract: GroES
Metabolic function and regulation of this protein in SubtiPathways: groES
MW, pI	10 kDa, 4.595
Gene length, protein length	282 bp, 94 aa
Immediate neighbours	ydiL, groEL
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

chaperones/ protein folding, heat shock proteins, essential genes, universally conserved proteins, most abundant proteins

This gene is a member of the following regulons

HrcA regulon

The gene

Basic information

• Locus tag: BSU06020

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU06020

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: groES chaperonin family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on Ser-36 PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • GroEL-GroES

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU06020

• Structure:

• UniProt: P28599

• KEGG entry: [3]

• E.C. number:

Additional information

universally conserved protein

Expression and regulation

• Operon: groES-groEL PubMed

• Expression browser: groES PubMed

• Sigma factor: SigA PubMed

• Regulation: induced by heat shock (HrcA) PubMed

• Regulatory mechanism: HrcA: transcription repression PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 4303 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 10235 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Wolfgang Schumann, Bayreuth University, Germany Homepage

References

Boumediene Soufi, Chanchal Kumar, Florian Gnad, Matthias Mann, Ivan Mijakovic, Boris Macek
Stable isotope labeling by amino acids in cell culture (SILAC) applied to quantitative proteomics of Bacillus subtilis.
J Proteome Res: 2010, 9(7);3638-46
[PubMed:20509597] [WorldCat.org] [DOI] (I p)

Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759] [WorldCat.org] [DOI] (P p)

A Schulz, W Schumann
hrcA, the first gene of the Bacillus subtilis dnaK operon encodes a negative regulator of class I heat shock genes.
J Bacteriol: 1996, 178(4);1088-93
[PubMed:8576042] [WorldCat.org] [DOI] (P p)

G Yuan, S L Wong
Isolation and characterization of Bacillus subtilis groE regulatory mutants: evidence for orf39 in the dnaK operon as a repressor gene in regulating the expression of both groE and dnaK.
J Bacteriol: 1995, 177(22);6462-8
[PubMed:7592421] [WorldCat.org] [DOI] (P p)

A Schmidt, M Schiesswohl, U Völker, M Hecker, W Schumann
Cloning, sequencing, mapping, and transcriptional analysis of the groESL operon from Bacillus subtilis.
J Bacteriol: 1992, 174(12);3993-9
[PubMed:1350777] [WorldCat.org] [DOI] (P p)

M Li, S L Wong
Cloning and characterization of the groESL operon from Bacillus subtilis.
J Bacteriol: 1992, 174(12);3981-92
[PubMed:1350776] [WorldCat.org] [DOI] (P p)