MurB

• Description: UDP-N-acetylenolpyruvoylglucosamine reductase
  
  

Gene name	murB
Synonyms	ylxC
Essential	yes PubMed
Product	UDP-N-acetylenolpyruvoylglucosamine reductase
Function	peptidoglycan precursor biosynthesis
Gene expression levels in SubtiExpress: murB
Metabolic function and regulation of this protein in SubtiPathways: murB
MW, pI	32 kDa, 6.087
Gene length, protein length	909 bp, 303 aa
Immediate neighbours	murG, divIB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, sporulation proteins, cell envelope stress proteins (controlled by SigM, V, W, X, Y), essential genes

This gene is a member of the following regulons

SigE regulon, SigM regulon, SpoIIID regulon

The gene

Basic information

• Locus tag: BSU15230

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU15230

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: UDP-N-acetylmuramate + NADP⁺ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH (according to Swiss-Prot)

• Protein family: FAD-binding PCMH-type domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU15230

• Structure: 2GQU (from Thermus caldophilus, 38% identity, 55% similarity) PubMed

• UniProt: P18579

• KEGG entry: [3]

• E.C. number: 1.1.1.158

Expression and regulation

• Operon:
  • murE-mraY-murD-spoVE-murG-murB-divIB-ylxW-ylxX-sbp PubMed
  • spoVE-murG-murB-divIB-ylxW-ylxX-sbp PubMed
  • murG-murB-divIB-ylxW-ylxX-sbp PubMed

• Expression browser: murB PubMed

• Sigma factor:
  • spoVE: SigE PubMed
  • murG: SigMPubMed

• Regulation:
  • expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell (SigE) PubMed

• Regulatory mechanism:
  • SpoIIID: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1522 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1652 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Adriano Henriques, Lisbon, Portugal homepage