Spo0F

• Description: phosphotransferase of the sporulation initiation phosphorelay
  
  

Gene name	spo0F
Synonyms
Essential	no
Product	phosphotransferase of the sporulation initiation phosphorelay
Function	initiation of sporulation
Gene expression levels in SubtiExpress: spo0F
Interactions involving this protein in SubtInteract: Spo0F
Function and regulation of this protein in SubtiPathways: spo0F
MW, pI	14 kDa, 4.697
Gene length, protein length	372 bp, 124 aa
Immediate neighbours	fbaA, ywjG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphorelay, phosphoproteins

This gene is a member of the following regulons

SigH regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU37130

Database entries

• BsubCyc: BSU37130

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on an Asp residue by KinA, KinB, KinC, KinD, or KinE
  • dephosphorylated by RapA, RapB, RapE, or RapH PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • Spo0F-Spo0B PubMed
  • Spo0F-Spo0E PubMed
  • RapA-Spo0F PubMed
  • RapB-Spo0F PubMed
  • RapE-Spo0F
  • RapH-Spo0F PubMed
  • KinA-Spo0F
  • KinB-Spo0F
  • KinC-Spo0F
  • KinD-Spo0F
  • KinE-Spo0F
  • YmcA-Spo0F PubMed
  • RapP-Spo0F, dephosphorylation of Spo0F PubMed
  • RapJ-Spo0F, dephosphorylation of Spo0F PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU37130

• Structure:
  • 1SRR (phosphatase resistant mutant)
  • 1F51 (complex with Spo0B)
  • 2JVK (Mutant L66A),
  • 2FSP
  • 3Q15 (RapH-Spo0F complex) PubMed

• UniProt: P06628

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spo0F PubMed

• Expression browser: spo0F PubMed

• Sigma factor: SigA PubMed, SigH PubMed

• Regulation:
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1508 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 750 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: