RapF

• Description: response regulator aspartate phosphatase, anti-activator protein, controls ComA activity
  
  

Gene name	rapF
Synonyms	ywhJ
Essential	no
Product	response regulator aspartate phosphatase
Function	control of ComA activity
Gene expression levels in SubtiExpress: rapF
Interactions involving this protein in SubtInteract: RapF
MW, pI	45 kDa, 5.18
Gene length, protein length	1143 bp, 381 aa
Immediate neighbours	ywhK, phrF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, protein modification, transcription factors and their control, quorum sensing

This gene is a member of the following regulons

CcpA regulon, ComA regulon

The gene

Basic information

• Locus tag: BSU37460

Database entries

• BsubCyc: BSU37460

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: control of ComA activity PubMed

• Protein family: RAP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: binding of PhrF to RapF results in a conformational change of RapF and prevents it from binding ComA PubMed

• Interactions:
  • RapF-ComA PubMed
  • RapF-PhrF PubMed

• Localization:

Database entries

• BsubCyc: BSU37460

• Structure:
  • 3ULQ (structure of the complex between the DNA-binding C-terminal domain of ComA with RapF) PubMed

• UniProt: P71002

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rapF-phrF PubMed

• Expression browser: rapF PubMed

• Sigma factor:

• Regulation: repressed by glucose (5-fold) (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • ComA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Melinda D Baker, Matthew B Neiditch
Structural basis of response regulator inhibition by a bacterial anti-activator protein.
PLoS Biol: 2011, 9(12);e1001226
[PubMed:22215984] [WorldCat.org] [DOI] (I p)

Natalia Comella, Alan D Grossman
Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis.
Mol Microbiol: 2005, 57(4);1159-74
[PubMed:16091051] [WorldCat.org] [DOI] (P p)

Cristina Bongiorni, Shu Ishikawa, Sophie Stephenson, Naotake Ogasawara, Marta Perego
Synergistic regulation of competence development in Bacillus subtilis by two Rap-Phr systems.
J Bacteriol: 2005, 187(13);4353-61
[PubMed:15968044] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

R S McQuade, N Comella, A D Grossman
Control of a family of phosphatase regulatory genes (phr) by the alternate sigma factor sigma-H of Bacillus subtilis.
J Bacteriol: 2001, 183(16);4905-9
[PubMed:11466295] [WorldCat.org] [DOI] (P p)