Difference between revisions of "SecA"

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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35300&redirect=T BSU35300]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/secA-prfB.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/secA-prfB.html]
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35300&redirect=T BSU35300]
  
 
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1TF5 1TF5] (open structure),  [http://www.rcsb.org/pdb/explore.do?structureId=2IBM 2IBM], [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3DL8 3DL8] (structure of the ([[SecY]]-[[SecE]]-[[SecG]])-[[SecA]] complex {{PubMed|18923516}}
 
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1TF5 1TF5] (open structure),  [http://www.rcsb.org/pdb/explore.do?structureId=2IBM 2IBM], [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3DL8 3DL8] (structure of the ([[SecY]]-[[SecE]]-[[SecG]])-[[SecA]] complex {{PubMed|18923516}}

Revision as of 14:55, 2 April 2014

  • Description: preprotein translocase subunit (ATPase), required for membrane targeting of DivIVA

Gene name secA
Synonyms div, div-341, ts-341
Essential yes PubMed
Product preprotein translocase subunit (ATPase)
Function protein secretion
Gene expression levels in SubtiExpress: secA
Interactions involving this protein in SubtInteract: SecA
Metabolic function and regulation of this protein in SubtiPathways:
Protein secretion
MW, pI 95 kDa, 5.34
Gene length, protein length 2523 bp, 841 aa
Immediate neighbours prfB, yvyD
Sequences Protein DNA DNA_with_flanks
Genetic context
SecA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SecA expression.png















Categories containing this gene/protein

protein secretion, essential genes, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU35300

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • ATP -> ADP + Pi + preprotein translocation
    • required for membrane targeting of DivIVA PubMed
  • Protein family: SecA family (according to Swiss-Prot)
  • Paralogous protein(s): none in Bacillus, some species have a paralogous secA gene named secA2 that has an altered substrate range

Extended information on the protein

  • Kinetic information:
  • Domains: nucleotide binding domain, preprotein binding domain, IRA2 domain, scaffold domain, wing domain, IRA1 domain, C-terminal domain
  • Modification:
  • Effectors of protein activity: anionic phospholipids, preprotein, SecY, signal peptides (even when added in trans) PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References