Difference between revisions of "GapB"
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=== Database entries === | === Database entries === | ||
+ | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29020&redirect=T BSU29020] | ||
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gapB-speD.html] | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gapB-speD.html] | ||
Line 93: | Line 94: | ||
=== Database entries === | === Database entries === | ||
+ | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29020&redirect=T BSU29020] | ||
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=3PRL 3PRL] (from ''B. halodurans'') | * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=3PRL 3PRL] (from ''B. halodurans'') |
Revision as of 14:29, 2 April 2014
- Description: glyceraldehyde-3-phosphate dehydrogenase, NADP-dependent, gluconeogenic enzyme, forms a transhydrogenation cycle with GapA for balancing of NADPH
Gene name | gapB |
Synonyms | ppc |
Essential | no |
Product | glyceraldehyde-3-phosphate dehydrogenase 2 |
Function | anabolic enzyme in gluconeogenesis |
Gene expression levels in SubtiExpress: gapB | |
Metabolic function and regulation of this protein in SubtiPathways: gapB | |
MW, pI | 37,3 kDa, 6.47 |
Gene length, protein length | 1020 bp, 340 amino acids |
Immediate neighbours | speD, ytcD |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU29020
Phenotypes of a mutant
Database entries
- BsubCyc: BSU29020
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H (according to Swiss-Prot)
- This reaction is part of the gluconeogenesis
- Protein family: glyceraldehyde-3-phosphate dehydrogenase family (according to Swiss-Prot)
- Paralogous protein(s): GapA
Extended information on the protein
- Kinetic information: Michaelis-Menten PubMed
- Domains:
- Nucleotid bindinge domain (12-13)
- 2x Glyceraldehyde 3-phosphate binding domain (151-153) & (210-211)
- Modification:
- Cofactor(s): NADP+ (preferentially) and NAD+ PubMed
- Effectors of protein activity:
- Localization: Cytoplasm (Homogeneous) PubMed
Database entries
- BsubCyc: BSU29020
- Structure: 3PRL (from B. halodurans)
- UniProt: O34425
- KEGG entry: [3]
- E.C. number: 1.2.1.59
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- Antibody:
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
Your additional remarks
References