TxpA

• Description: toxic peptide
  
  

Gene name	txpA
Synonyms	yqcR, yqdB
Essential	no
Product	toxic peptide
Function	toxin
Gene expression levels in SubtiExpress: txpA
MW, pI	6 kDa, 8.139
Gene length, protein length	177 bp, 59 aa
Immediate neighbours	yqbN/2, ratA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

toxins, antitoxins and immunity against toxins, Skin element, membrane proteins

This gene is a member of the following regulons

ratA regulon

The gene

Basic information

• Locus tag: BSU26050

Database entries

• BsubCyc: BSU26050

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane

Database entries

• BsubCyc: BSU26050

• Structure:

• UniProt: P54373

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: txpA PubMed

• Expression browser: txpA PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:
  • expression is prevented by the ratA antisense RNA PubMed
  • the txpA mRNA-RatA RNA duplex is degraded by RNase III PubMed

• Additional information:
  • next to yonT, the txpA mRNA has the strongest Shine-Dalgarno region in B. subtilis PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Richard Losick, Harvard University, Cambridge, USA Homepage

References

Reviews

Fabian M Commichau, Jörg Stülke
A mystery unraveled: essentiality of RNase III in Bacillus subtilis is caused by resident prophages.
PLoS Genet: 2012, 8(12);e1003199
[PubMed:23300472] [WorldCat.org] [DOI] (I p)

Original publications

Durand S, Gilet L, Condon C  
The essential function of B. subtilis RNase III is to silence foreign toxin genes. 
PLoS Genet. 2012 8(12): e1003181.
PubMed:23300471

Roula Daou-Chabo, Nathalie Mathy, Lionel Bénard, Ciarán Condon
Ribosomes initiating translation of the hbs mRNA protect it from 5'-to-3' exoribonucleolytic degradation by RNase J1.
Mol Microbiol: 2009, 71(6);1538-50
[PubMed:19210617] [WorldCat.org] [DOI] (I p)

Jessica M Silvaggi, John B Perkins, Richard Losick
Small untranslated RNA antitoxin in Bacillus subtilis.
J Bacteriol: 2005, 187(19);6641-50
[PubMed:16166525] [WorldCat.org] [DOI] (P p)