CotW

• Description: outer spore coat protein (insoluble fraction), formation of the spore crust
  
  

Gene name	cotW
Synonyms
Essential	no
Product	spore coat protein (insoluble fraction)
Function	resistance of the spore
Gene expression levels in SubtiExpress: cotW
Interactions involving this protein in SubtInteract: CotW
MW, pI	12 kDa, 9.955
Gene length, protein length	315 bp, 105 aa
Immediate neighbours	cotX, cotV
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

GerE regulon, SigE regulon SigK regulon, SpoIIID regulon

The gene

Basic information

• Locus tag: BSU11770

Database entries

• BsubCyc: BSU11770

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • CotV-CotW PubMed

• Localization:
  • spore crust, localization depends on CotX, CotY, and CotZ PubMed

Database entries

• BsubCyc: BSU11770

• Structure:

• UniProt: Q08310

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cotV-cotW-cotX PubMed

• Expression browser: cotW PubMed

• Sigma factor: SigE PubMed , SigK PubMed

• Regulation:
  • expressed during sporulation in the mother cell (SigE, SigK, GerE, SpoIIID) PubMed
  • repressed by casamino acids PubMed

• Regulatory mechanism:
  • GerE: transcription activation PubMed
  • SpoIIID: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Peter T McKenney, Adam Driks, Patrick Eichenberger
The Bacillus subtilis endospore: assembly and functions of the multilayered coat.
Nat Rev Microbiol: 2013, 11(1);33-44
[PubMed:23202530] [WorldCat.org] [DOI] (I p)