Difference between revisions of "FabHA"

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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU11330&redirect=T BSU11330]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fabHAF.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fabHAF.html]
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU11330&redirect=T BSU11330]
  
 
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1EBL 1EBL] (FabH from ''E. coli'') {{PubMed|10673437}}  
 
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1EBL 1EBL] (FabH from ''E. coli'') {{PubMed|10673437}}  

Revision as of 13:24, 2 April 2014

  • Description: beta-ketoacyl-acyl carrier protein synthase III, principal condensing enzyme responsible for the initiation of fatty acid synthesis in non-stressed B. subtilis cells

Gene name fabHA
Synonyms yjaX , fabH1
Essential no
Product beta-ketoacyl-acyl carrier protein synthase III
Function fatty acid biosynthesis
Gene expression levels in SubtiExpress: fabHA
Metabolic function and regulation of this protein in SubtiPathways:
fabHA
MW, pI 33 kDa, 5.045
Gene length, protein length 936 bp, 312 aa
Immediate neighbours yjzB, fabF
Sequences Protein DNA DNA_with_flanks
Genetic context
FabHA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FabHA expression.png















Categories containing this gene/protein

biosynthesis of lipids

This gene is a member of the following regulons

FapR regulon

The gene

Basic information

  • Locus tag: BSU11330

Phenotypes of a mutant

  • significant increase in the proportion of straight-chain fatty acids with a concomitant increase in 31:0-carbon phosphatidylethanolamine species PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacyl-[acyl-carrier-protein] + CoA + CO2 (according to Swiss-Prot)
  • Protein family: fabH family (according to Swiss-Prot)
  • Paralogous protein(s): FabHB, one of the two proteins has to be present for viability PubMed

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

  • affinity for butyryl-CoA, but prefers acetyl-CoA in fatty acid biosynthesis PubMed

Expression and regulation

  • Regulation:
    • expressed when the cells experience a lack of malonyl-CoA (FapR) PubMed
    • inhibited by cerulenin PubMed
    • induced upon fatty acid biosynthesis inhibition PubMed
    • expression is reduced when SigW is activated (by alkaline shock, polymyxin B, vancomycin, cephalosporin C, D-cycloserine, and triton X-100) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original Publications