Difference between revisions of "PurB"

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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU06440&redirect=T BSU06440]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/purEKBCSQLFMNHD.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/purEKBCSQLFMNHD.html]
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU06440&redirect=T BSU06440]
  
 
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1F1O 1F1O]
 
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1F1O 1F1O]

Revision as of 13:07, 2 April 2014

  • Description: adenylsuccinate lyase

Gene name purB
Synonyms
Essential no
Product adenylsuccinate lyase
Function purine biosynthesis
Gene expression levels in SubtiExpress: purB
Metabolic function and regulation of this protein in SubtiPathways:
purB
MW, pI 49 kDa, 5.822
Gene length, protein length 1293 bp, 431 aa
Immediate neighbours purK, purC
Sequences Protein DNA DNA_with_flanks
Genetic context
PurB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PurB expression.png















Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, most abundant proteins

This gene is a member of the following regulons

G-box, PurR regulon

The gene

Basic information

  • Locus tag: BSU06440

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: N(6)-(1,2-dicarboxyethyl)AMP = fumarate + AMP (according to Swiss-Prot)
  • Protein family: Adenylosuccinate lyase subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Regulation:
    • repressed in the presence of purine nucleotides (PurR) PubMed
    • repressed in the presence of adenine or adenosine(PurR) PubMed
    • repressed in the presence of guanine(G-box) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References