Difference between revisions of "GlmM"
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU01770&redirect=T BSU01770] | ||
* '''DBTBS entry:''' no entry | * '''DBTBS entry:''' no entry | ||
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU01770&redirect=T BSU01770] | ||
* '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3PDK 3PDK] (from ''B. anthracis'') {{PubMed|21685296}} | * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3PDK 3PDK] (from ''B. anthracis'') {{PubMed|21685296}} | ||
Revision as of 12:52, 2 April 2014
- Description: phosphoglucosamine mutase, required for cell wall synthesis
| Gene name | glmM |
| Synonyms | ybbT |
| Essential | yes PubMed |
| Product | phosphoglucosamine mutase, required for cell wall synthesis |
| Function | cell wall synthesis |
| Gene expression levels in SubtiExpress: glmM | |
| Metabolic function and regulation of this protein in SubtiPathways: glmM | |
| MW, pI | 48 kDa, 4.677 |
| Gene length, protein length | 1344 bp, 448 aa |
| Immediate neighbours | cdaR, glmS |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
cell wall synthesis, biosynthesis of cell wall components, essential genes, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU01770
Phenotypes of a mutant
essential PubMed
Database entries
- BsubCyc: BSU01770
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate (according to Swiss-Prot)
- Protein family: phosphohexose mutase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU01770
- UniProt: O34824
- KEGG entry: [2]
- E.C. number: 5.4.2.10
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
Biological materials
- Mutant:
- Expression vector:
- pGP400: (expression in B. subtilis in pBQ200), available in Jörg Stülke's lab
- pGP1401: (expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
- pGP1402: (cloning vector for glmM (GlmM S100A) in E. coli, in pBlueskript KS), available in Jörg Stülke's lab
- pGP1403: (expression of GlmM (S100A) in B. subtilis in pBQ200), available in Jörg Stülke's lab
- pGP1405: (expression, purification of GlmM (S100A) in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
- lacZ fusion:
- GP1340 (cat) based on pAC6, available in Jörg Stülke's lab
- GFP fusion:
- GP1382 glmM-gfp ermC (based on pGP1080), available in Jörg Stülke's lab
- two-hybrid system:
- pGP2576 (glmM in p25N), available in Jörg Stülke's lab
- FLAG-tag construct:
- GP1380 lacA::cdaR-Strep aphA3 glmM-3xFLAG ermC (based on pGP1460 and pGP1087), available in Jörg Stülke's lab
- Strep-tag construct:
- GP1388 lacA::glmM-Strep aphA3 cdaA-3xFLAG ermC (based on pGP1460 and pGP1087), available in Jörg Stülke's lab
- Antibody: available in Jörg Stülke's lab
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
