Difference between revisions of "DarA"
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yaaQ_39871_40200_1 yaaQ] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yaaQ_39871_40200_1 yaaQ] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
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* '''Mutant:''' | * '''Mutant:''' | ||
| − | ** GP1712 (''yaaQ''::''cat''), available in [[Jörg Stülke]]'s lab | + | ** GP1712 (''[[yaaQ]]''::''cat''), available in [[Jörg Stülke]]'s lab |
| − | ** GP1713 (''yaaQ- | + | ** GP1713 (''[[yaaQ]]-[[yaaR]]''::''cat''), available in [[Jörg Stülke]]'s lab |
* '''Expression vector:''' | * '''Expression vector:''' | ||
| − | **pGP2601: | + | **pGP2601: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]], available in [[Jörg Stülke]]'s lab |
**pGP2603: expression of ''yaaQ''-Strep by [[pGP382]] in ''B. subtilis'' suitable for [[SPINE]], available in [[Jörg Stülke]]'s lab | **pGP2603: expression of ''yaaQ''-Strep by [[pGP382]] in ''B. subtilis'' suitable for [[SPINE]], available in [[Jörg Stülke]]'s lab | ||
Revision as of 11:59, 14 March 2014
- Description: unknown
| Gene name | yaaQ |
| Synonyms | |
| Essential | |
| Product | unknown |
| Function | unknown |
| Gene expression levels in SubtiExpress: yaaQ | |
| MW, pI | 11 kDa, 6.088 |
| Gene length, protein length | 327 bp, 109 aa |
| Immediate neighbours | tmk, yaaR |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU00290
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Interactions:
- forms homo-trimers (see 3M05)
Database entries
- Structure:
- 3M05 (the protein from Pediococcus pentosaceus, 50% identity)
- UniProt: P37538
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP1712 (yaaQ::cat), available in Jörg Stülke's lab
- GP1713 (yaaQ-yaaR::cat), available in Jörg Stülke's lab
- Expression vector:
- pGP2601: IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab
- pGP2603: expression of yaaQ-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849]
[WorldCat.org]
[DOI]
(I p)
