Difference between revisions of "KtrA"

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(Expression and regulation)
(Biological materials)
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* '''Expression vector:'''
 
* '''Expression vector:'''
 +
**pGP2594: (expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
 
          
 
          
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''

Revision as of 09:20, 14 March 2014

  • Description: high affinity potassium transporter KtrA-KtrB, peripheric membrane component (proton symport)

Gene name ktrA
Synonyms yuaA
Essential no
Product high affinity potassium transporter KtrA-KtrB,
peripheric membrane component (proton symport)
Function potassium uptake
Gene expression levels in SubtiExpress: ktrA
Interactions involving this protein in SubtInteract: KtrA
Metabolic function and regulation of this protein in SubtiPathways:
Metal ion homeostasis, ktrA
MW, pI 24 kDa, 5.981
Gene length, protein length 666 bp, 222 aa
Immediate neighbours bslA, ktrB
Sequences Protein DNA DNA_with_flanks
Genetic context
YuaA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
KtrA expression.png















Categories containing this gene/protein

transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), coping with hyper-osmotic stress, membrane proteins

This gene is a member of the following regulons

YdaO riboswitch

The gene

Basic information

  • Locus tag: BSU31090

Phenotypes of a mutant

  • The ktrA-ktrB mutant of B. subtilis NCIB3610 is reduced in sliding (dendritic spreading) PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): KtrC

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:
    • the protein binds c-di-AMP PubMed

Database entries

  • KEGG entry: [4]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
    • expression is controlled via termination antitermination by the ydaO riboswitch, expression is switched off upon binding of c-di-AMP PubMed
  • Additional information:

Biological materials

  • Expression vector:
    • pGP2594: (expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage

Your additional remarks

References