Difference between revisions of "Pgk"
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{{SubtiWiki category|[[carbon core metabolism]]}}, | {{SubtiWiki category|[[carbon core metabolism]]}}, | ||
{{SubtiWiki category|[[phosphoproteins]]}}, | {{SubtiWiki category|[[phosphoproteins]]}}, | ||
| − | {{SubtiWiki category|[[universally conserved proteins]]}} | + | {{SubtiWiki category|[[universally conserved proteins]]}}, |
| + | [[most abundant proteins]] | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| Line 64: | Line 65: | ||
=== Additional information=== | === Additional information=== | ||
| − | |||
| − | |||
=The protein= | =The protein= | ||
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* '''Kinetic information:''' Two Substrate Reversible Michaelis-Menten [http://www.ncbi.nlm.nih.gov/pubmed/7154941 PubMed] | * '''Kinetic information:''' Two Substrate Reversible Michaelis-Menten [http://www.ncbi.nlm.nih.gov/pubmed/7154941 PubMed] | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
** nucleotide binding domain (ATP) (350–353) | ** nucleotide binding domain (ATP) (350–353) | ||
** 2x substrate binding domain (21–23), (59–62) | ** 2x substrate binding domain (21–23), (59–62) | ||
| Line 86: | Line 85: | ||
* '''Modification:''' phosphorylation on Ser-183 AND Thr-299 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed] | * '''Modification:''' phosphorylation on Ser-183 AND Thr-299 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed] | ||
| − | * ''' | + | * '''[[Cofactors]]:''' Mg2+ or Mn2+ [http://www.ncbi.nlm.nih.gov/pubmed/7154941 PubMed] |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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* '''Additional information:''' | * '''Additional information:''' | ||
| + | ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}} | ||
=Biological materials = | =Biological materials = | ||
| Line 155: | Line 155: | ||
=References= | =References= | ||
| − | <pubmed> 16479537, 12850135 , 17726680, 11489127 ,17505547 , 17218307 7154941 12682299 23420519</pubmed> | + | <pubmed> 16479537, 12850135 , 17726680, 11489127 ,17505547 , 17218307 7154941 12682299 23420519 15378759</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 14:37, 5 March 2014
- Description: phosphoglycerate kinase, glycolytic/ gluconeogenic enzyme, universally conserved protein
| Gene name | pgk |
| Synonyms | |
| Essential | No |
| Product | phosphoglycerate kinase |
| Function | enzyme in glycolysis/ gluconeogenesis |
| Gene expression levels in SubtiExpress: pgk | |
| Metabolic function and regulation of this protein in SubtiPathways: pgk | |
| MW, pI | 42,0 kDa, 4.77 |
| Gene length, protein length | 1182 bp, 394 amino acids |
| Immediate neighbours | tpi, gapA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
ATP synthesis, carbon core metabolism, phosphoproteins, universally conserved proteins, most abundant proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU33930
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + 3-phospho-D-glycerate = ADP + 1,3-bisphosphoglycerate (according to Swiss-Prot)
- Protein family: phosphoglycerate kinase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: Two Substrate Reversible Michaelis-Menten PubMed
- Domains:
- nucleotide binding domain (ATP) (350–353)
- 2x substrate binding domain (21–23), (59–62)
- Effectors of protein activity:
- Inhibited by Co2+, NDP and NMP PubMed
- Localization: cytoplasm (according to Swiss-Prot), Cytoplasm (Homogeneous) PubMed
Database entries
- Structure: 1PHP (from Geobacillus stearothermophilus)
- UniProt: P40924
- KEGG entry: [3]
- E.C. number: 2.7.2.3
Additional information
- extensive information on the structure and enzymatic properties of Pgk can be found at Proteopedia
Expression and regulation
- Regulation:
- Additional information:
- belongs to the 100 most abundant proteins PubMed
Biological materials
- Mutant:
- GP699 (pgk::cat), available in Jörg Stülke's lab, PubMedlab
- GP707 (pgk::erm), available in Jörg Stülke's lab, PubMed
- Expression vector:
- pGP1102 (N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
- pGP95 (N-terminal Strep-tag, in pGP172), available in Jörg Stülke's lab
- pGP91 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Jörg Stülke's lab
- pGP1513 (expression in B. subtilis, in pBQ200), available in Jörg Stülke's lab
- lacZ fusion: pGP514 (in pAC6), a series of promoter deletion variants is also available in pAC6, available in Jörg Stülke's lab
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available iin Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
