Difference between revisions of "SpoIIID"

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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:''' two DNA-binding regions: HTH at the N-terminus and a basic region near the C-terminus {{PubMed|20061473}}
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* '''[[Domains]]:''' two DNA-binding regions: HTH at the N-terminus and a basic region near the C-terminus {{PubMed|20061473}}
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
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* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spoIIID_3748421_3748702_-1 spoIIID] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spoIIID_3748421_3748702_-1 spoIIID] {{PubMed|22383849}}
  
* '''Sigma factor:''' [[SigE]] {{PubMed|9023218}}  
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* '''[[Sigma factor]]:''' [[SigE]] {{PubMed|9023218}}  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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=References=
 
=References=
'''Additional publications:''' {{PubMed|22463703}}
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<pubmed>15383836,1469717,7896717,1900494,8231808,9023218,2112673,1691789,10788508,1744038,7966271,2514119,1518043,17693505,9006059, 20061473 17890309 17693499 7836311 24584501 22463703</pubmed>
<pubmed>15383836,1469717,7896717,1900494,8231808,9023218,2112673,1691789,10788508,1744038,7966271,2514119,1518043,17693505,9006059, 20061473 17890309 17693499 7836311</pubmed>
 
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 17:22, 4 March 2014

  • Description: transcriptional regulator (repressor or activator) of a subset of sigma-E-dependent genes

Gene name spoIIID
Synonyms
Essential no
Product transcriptional regulator
Function regulation of mother cell gene expression
Gene expression levels in SubtiExpress: spoIIID
Metabolic function and regulation of this protein in SubtiPathways:
spoIIID
MW, pI 10 kDa, 9.496
Gene length, protein length 279 bp, 93 aa
Immediate neighbours mbl, usd
Sequences Protein DNA DNA_with_flanks
Genetic context
SpoIIID context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SpoIIID expression.png















Categories containing this gene/protein

transcription factors and their control, sporulation proteins

This gene is a member of the following regulons

SigE regulon

The SpoIIID regulon

The gene

Basic information

  • Locus tag: BSU36420

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: binds its DNA targets as a monomeric protein (possible due to the two DNA-binding motifs) PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: two DNA-binding regions: HTH at the N-terminus and a basic region near the C-terminus PubMed
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 2L0K (complex with DNA)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed early during sporulation in the mother cell (SigE) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Bin Chen, Paul Himes, Yu Liu, Yang Zhang, Zhenwei Lu, Aizhuo Liu, Honggao Yan, Lee Kroos
Structure of bacterial transcription factor SpoIIID and evidence for a novel mode of DNA binding.
J Bacteriol: 2014, 196(12);2131-42
[PubMed:24584501] [WorldCat.org] [DOI] (I p)

Sarah E Ebmeier, Irene S Tan, Katie Rose Clapham, Kumaran S Ramamurthi
Small proteins link coat and cortex assembly during sporulation in Bacillus subtilis.
Mol Microbiol: 2012, 84(4);682-96
[PubMed:22463703] [WorldCat.org] [DOI] (I p)

Paul Himes, Steven J McBryant, Lee Kroos
Two regions of Bacillus subtilis transcription factor SpoIIID allow a monomer to bind DNA.
J Bacteriol: 2010, 192(6);1596-606
[PubMed:20061473] [WorldCat.org] [DOI] (I p)

Lijuan Wang, John Perpich, Adam Driks, Lee Kroos
One perturbation of the mother cell gene regulatory network suppresses the effects of another during sporulation of Bacillus subtilis.
J Bacteriol: 2007, 189(23);8467-73
[PubMed:17890309] [WorldCat.org] [DOI] (I p)

Chris Guillot, Charles P Moran
Essential internal promoter in the spoIIIA locus of Bacillus subtilis.
J Bacteriol: 2007, 189(20);7181-9
[PubMed:17693505] [WorldCat.org] [DOI] (P p)

Lijuan Wang, John Perpich, Adam Driks, Lee Kroos
Maintaining the transcription factor SpoIIID level late during sporulation causes spore defects in Bacillus subtilis.
J Bacteriol: 2007, 189(20);7302-9
[PubMed:17693499] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

H Ichikawa, L Kroos
Combined action of two transcription factors regulates genes encoding spore coat proteins of Bacillus subtilis.
J Biol Chem: 2000, 275(18);13849-55
[PubMed:10788508] [WorldCat.org] [DOI] (P p)

A Decatur, M T McMurry, B N Kunkel, R Losick
Translation of the mRNA for the sporulation gene spoIIID of Bacillus subtilis is dependent upon translation of a small upstream open reading frame.
J Bacteriol: 1997, 179(4);1324-8
[PubMed:9023218] [WorldCat.org] [DOI] (P p)

B Zhang, R A Daniel, J Errington, L Kroos
Bacillus subtilis SpoIIID protein binds to two sites in the spoVD promoter and represses transcription by sigmaE RNA polymerase.
J Bacteriol: 1997, 179(3);972-5
[PubMed:9006059] [WorldCat.org] [DOI] (P p)

R Halberg, V Oke, L Kroos
Effects of Bacillus subtilis sporulation regulatory protein SpoIIID on transcription by sigma K RNA polymerase in vivo and in vitro.
J Bacteriol: 1995, 177(7);1888-91
[PubMed:7896717] [WorldCat.org] [DOI] (P p)

Y Abhayawardhane, G C Stewart
Bacillus subtilis possesses a second determinant with extensive sequence similarity to the Escherichia coli mreB morphogene.
J Bacteriol: 1995, 177(3);765-73
[PubMed:7836311] [WorldCat.org] [DOI] (P p)

R Halberg, L Kroos
Sporulation regulatory protein SpoIIID from Bacillus subtilis activates and represses transcription by both mother-cell-specific forms of RNA polymerase.
J Mol Biol: 1994, 243(3);425-36
[PubMed:7966271] [WorldCat.org] [DOI] (P p)

P A Levin, N Fan, E Ricca, A Driks, R Losick, S Cutting
An unusually small gene required for sporulation by Bacillus subtilis.
Mol Microbiol: 1993, 9(4);761-71
[PubMed:8231808] [WorldCat.org] [DOI] (P p)

R Halberg, L Kroos
Fate of the SpoIIID switch protein during Bacillus subtilis sporulation depends on the mother-cell sigma factor, sigma K.
J Mol Biol: 1992, 228(3);840-9
[PubMed:1469717] [WorldCat.org] [DOI] (P p)

L Zheng, R Halberg, S Roels, H Ichikawa, L Kroos, R Losick
Sporulation regulatory protein GerE from Bacillus subtilis binds to and can activate or repress transcription from promoters for mother-cell-specific genes.
J Mol Biol: 1992, 226(4);1037-50
[PubMed:1518043] [WorldCat.org] [DOI] (P p)

K M Tatti, C H Jones, C P Moran
Genetic evidence for interaction of sigma E with the spoIIID promoter in Bacillus subtilis.
J Bacteriol: 1991, 173(24);7828-33
[PubMed:1744038] [WorldCat.org] [DOI] (P p)

S Cutting, A Driks, R Schmidt, B Kunkel, R Losick
Forespore-specific transcription of a gene in the signal transduction pathway that governs Pro-sigma K processing in Bacillus subtilis.
Genes Dev: 1991, 5(3);456-66
[PubMed:1900494] [WorldCat.org] [DOI] (P p)

L B Zheng, R Losick
Cascade regulation of spore coat gene expression in Bacillus subtilis.
J Mol Biol: 1990, 212(4);645-60
[PubMed:1691789] [WorldCat.org] [DOI] (P p)

C M Stevens, J Errington
Differential gene expression during sporulation in Bacillus subtilis: structure and regulation of the spoIIID gene.
Mol Microbiol: 1990, 4(4);543-51
[PubMed:2112673] [WorldCat.org] [DOI] (P p)

B Kunkel, L Kroos, H Poth, P Youngman, R Losick
Temporal and spatial control of the mother-cell regulatory gene spoIIID of Bacillus subtilis.
Genes Dev: 1989, 3(11);1735-44
[PubMed:2514119] [WorldCat.org] [DOI] (P p)