Difference between revisions of "Pyk"
Line 79: | Line 79: | ||
=== Extended information on the protein === | === Extended information on the protein === | ||
− | * '''Kinetic information:''' | + | * '''Kinetic information:''' |
+ | ** allosteric Regulation [http://www.ncbi.nlm.nih.gov/pubmed/3711058 PubMed] | ||
+ | ** specific activity: 0.231 µmol min<sup>-1</sup> (mg protein)<sup>-1</sup> {{PubMed|24571712}} | ||
− | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' phosphorylation on Ser36 [http://www.ncbi.nlm.nih.gov/pubmed/17218307 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed], phosphorylation on Ser536 or Ser546 [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed], please note that the Ser is not on position 536 but rather at 538 | * '''Modification:''' phosphorylation on Ser36 [http://www.ncbi.nlm.nih.gov/pubmed/17218307 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed], phosphorylation on Ser536 or Ser546 [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed], please note that the Ser is not on position 536 but rather at 538 | ||
− | * ''' | + | * '''[[Cofactors]]:''' Mg2+, K+ |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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=References= | =References= | ||
− | <pubmed>17726680 16493705 11489127 17505547 10966427 17218307 3711058 4623707 21821766 22846916 23420519 24158146 </pubmed> | + | <pubmed>17726680 16493705 11489127 17505547 10966427 17218307 3711058 4623707 21821766 22846916 23420519 24158146 24571712</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 16:32, 28 February 2014
- Description: pyruvate kinase, glycolytic enzyme
Gene name | pyk |
Synonyms | pykA |
Essential | no |
Product | pyruvate kinase |
Function | catabolic enzyme in glycolysis |
Gene expression levels in SubtiExpress: pyk | |
Metabolic function and regulation of this protein in SubtiPathways: pyk | |
MW, pI | 61,9 kDa, 4.88 |
Gene length, protein length | 1755 bp, 585 amino acids |
Immediate neighbours | ytzA, pfkA |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
ATP synthesis, carbon core metabolism, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU29180
Phenotypes of a mutant
Unable to grow with non-PTS carbohydrates (such as glucitol or glycerol) as single carbon source.
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
- A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ADP + phosphoenolpyruvate --> ATP + pyruvate
- The reaction is irreversible under physiological conditions
- Protein family: PEP-utilizing enzyme family (according to Swiss-Prot) pyruvate kinase family, (C-terminal section: PEP-utilizing enzyme family)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification: phosphorylation on Ser36 PubMed, PubMed, phosphorylation on Ser536 or Ser546 PubMed, please note that the Ser is not on position 536 but rather at 538
- Cofactors: Mg2+, K+
- Effectors of protein activity:
- Localization: cytoplasm PubMed
Database entries
- Structure: 2E28 (Geobacillus stearothermophilus)
- UniProt: P80885
- KEGG entry: [3]
- E.C. number: 2.7.1.40
Additional information
The enzyme is a tetramer with four active sites PubMed
Expression and regulation
- Regulation:
- twofold induced by glucose PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP589 (pyk::cat), available in Jörg Stülke's lab, PubMed
- GP600 (pyk::erm), available in Jörg Stülke's lab, PubMed
- Expression vector:
- expression in E. coli, N-terminal His-tag: pGP1100 (in pWH844), available in Jörg Stülke's lab
- expression in B. subtilis, native protein: pGP1411 (in pBQ200), available in Jörg Stülke's lab
- expression in B. subtilis, N-terminal Strep-tag: pGP1409 (in pGP380), available in Jörg Stülke's lab
- expression in B. subtilis, C-terminal Strep-tag: pGP1410 (in pGP382), available in Jörg Stülke's lab
- lacZ fusion: see pfkA
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Jörg Stülke, University of Göttingen, Germany Homepage
Your additional remarks
References