Difference between revisions of "CwlT"
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
| + | ** N-terminal domain is an N-acetylmuramidase that cleaves the linkage between N-acetylmuramic acid and N-acetylglucosamine {{PubMed|22383849}} | ||
| + | ** C-terminal endopeptidase domain cleaves the bond between D-γ-glutamate and meso-diaminopimelic acid | ||
* '''Modification:''' | * '''Modification:''' | ||
Revision as of 18:09, 18 February 2014
- Description: cell wall hydrolase, C-terminal domain hydrolyzes bond between D-Glu and m-DAP
| Gene name | cwlT |
| Synonyms | yddH |
| Essential | |
| Product | cell wall hydrolase |
| Function | cell wall metabolism |
| Gene expression levels in SubtiExpress: cwlT | |
| MW, pI | 36 kDa, 8.632 |
| Gene length, protein length | 987 bp, 329 aa |
| Immediate neighbours | yddG, yddI |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
cell wall degradation/ turnover
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU04970
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: nlpC/p60 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- N-terminal domain is an N-acetylmuramidase that cleaves the linkage between N-acetylmuramic acid and N-acetylglucosamine PubMed
- C-terminal endopeptidase domain cleaves the bond between D-γ-glutamate and meso-diaminopimelic acid
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P96645
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- induced by mitomycin C PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Tyler DeWitt, Alan D Grossman
The bifunctional cell wall hydrolase CwlT is needed for conjugation of the integrative and conjugative element ICEBs1 in Bacillus subtilis and B. anthracis.
J Bacteriol: 2014, 196(8);1588-96
[PubMed:24532767]
[WorldCat.org]
[DOI]
(I p)
Tatsuya Fukushima, Toshihiko Kitajima, Hiroyuki Yamaguchi, Qin Ouyang, Kazumi Furuhata, Hiroki Yamamoto, Toshio Shida, Junichi Sekiguchi
Identification and characterization of novel cell wall hydrolase CwlT: a two-domain autolysin exhibiting n-acetylmuramidase and DL-endopeptidase activities.
J Biol Chem: 2008, 283(17);11117-25
[PubMed:18305117]
[WorldCat.org]
[DOI]
(P p)
