Difference between revisions of "SpoVS"
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spoVS_1769935_1770195_1 spoVS] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spoVS_1769935_1770195_1 spoVS] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' [[SigH]] {{PubMed|7559352}} | + | * '''[[Sigma factor]]:''' [[SigH]] {{PubMed|7559352}} |
* '''Regulation:''' | * '''Regulation:''' | ||
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=Biological materials = | =Biological materials = | ||
| − | * '''Mutant:''' GP1601 (cat), available in [[Stülke]] lab | + | * '''Mutant:''' GP1601 (cat), available in [[ Jörg Stülke]]'s lab |
* '''Expression vector:''' | * '''Expression vector:''' | ||
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* '''GFP fusion:''' | * '''GFP fusion:''' | ||
| − | * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab | + | * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[ Jörg Stülke]]'s lab |
* '''Antibody:''' | * '''Antibody:''' | ||
Revision as of 10:25, 31 January 2014
- Description: required for dehydratation of the spore core and assembly of the coat, mutation increases SigD-dependent gene expression, might act via SinR
| Gene name | spoVS |
| Synonyms | |
| Essential | no |
| Product | unknown |
| Function | spore coat assembly, spore core dehydratation |
| Gene expression levels in SubtiExpress: spoVS | |
| MW, pI | 8 kDa, 6.475 |
| Gene length, protein length | 258 bp, 86 aa |
| Immediate neighbours | ymdB, tdh |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU16980
Phenotypes of a mutant
increased SigD-dependent gene expression, this might occur via SinR PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P45693
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
- The mRNA has a long 5' leader region. This may indicate RNA-based regulation PubMed
Biological materials
- Mutant: GP1601 (cat), available in Jörg Stülke's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796]
[WorldCat.org]
[DOI]
(I p)
Daniel J Rigden, Michael Y Galperin
Sequence analysis of GerM and SpoVS, uncharacterized bacterial 'sporulation' proteins with widespread phylogenetic distribution.
Bioinformatics: 2008, 24(16);1793-7
[PubMed:18562273]
[WorldCat.org]
[DOI]
(I p)
Ana R Perez, Angelica Abanes-De Mello, Kit Pogliano
Suppression of engulfment defects in bacillus subtilis by elevated expression of the motility regulon.
J Bacteriol: 2006, 188(3);1159-64
[PubMed:16428420]
[WorldCat.org]
[DOI]
(P p)
O Resnekov, A Driks, R Losick
Identification and characterization of sporulation gene spoVS from Bacillus subtilis.
J Bacteriol: 1995, 177(19);5628-35
[PubMed:7559352]
[WorldCat.org]
[DOI]
(P p)
